LDLR Antibody (EP1553Y)

Product Discontinued

LDLR Antibody (EP1553Y) Summary

• Immunogen: A synthetic peptide corresponding to residues on the C-terminus of human LDL receptor was used as an immunogen.
• Specificity: This antibody detects both isoforms of LDL receptor.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: LDLR
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:70
  • Immunocytochemistry/ Immunofluorescence 1:100-250
  • Immunohistochemistry 1:100 - 1:250
  • Immunohistochemistry-Paraffin 1:100 - 1:250
  • Immunoprecipitation 1:50
  • Western Blot 1:1000-10000
• Application Notes: In Western blot this antibody detects a band at approximately 140 kDa. This antibody can also be used in Co-IP (PMID: 22128165).
• Control:

  LDLR Overexpression Lysate

Reactivity Notes

Rat and Mouse cross-reactivity tested by western blot only. Rat reactivity reported in scientific literature (PMID: 25664679)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for LDLR Antibody (EP1553Y)

• FH
• FHC
• LDL R
• LDL receptor
• LDLCQ2
• LDLR
• low density lipoprotein receptor
• low-density lipoprotein receptor class A domain-containing protein 3
• low-density lipoprotein receptor

Background

The low density lipoprotein (LDL) receptor system coordinates the metabolism of cholesterol, an essential component of the plasma membrane of all mammalian cells. Study of this system has led to an enhanced understanding of the cellular basis of cholesterol homeostasis. It has also brought into focus an important mechanism of metabolic regulation--the process of receptor-mediated endocytosis (1). Data suggest that the juxtamembranous region of the cytoplasmic domain participates in protein:protein interactions that allow the low density lipoprotein receptor to cluster in coated pits (2). It has been shown that the family of LDL receptors may serve as viral receptors. Endocytosis of the Flaviviridae viruses, hepatitis C virus, GB virus C/hepatitis G virus, and bovine viral diarrheal virus (BVDV) was shown to be mediated by LDL receptors on cultured cells (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LDLR Antibody (NB110-57162)(16)

Publications using NB110-57162

• Hu PA, Chen CH, Guo BC et al. Bromelain Confers Protection against the Non-Alcoholic Fatty Liver Disease in Male C57bl/6 Mice Nutrients 2020-05-18 [PMID: 32443556]
• Picard C, Poirier A, Belanger S et al. Proprotein convertase subtilisin/kexin type 9 (PCSK9) in Alzheimer's disease: A genetic and proteomic multi-cohort study PLoS ONE 2019-08-22 [PMID: 31437157] (ELISA, Human)
• Pennanen P, Syvala H, Blauer M et al. The effects of metformin and simvastatin on the growth of LNCaP and RWPE-1 prostate epithelial cell lines. Eur. J. Pharmacol. 2016-06-21 [PMID: 27341997]
• Pincon A, Coulombe JD, Chouinard-Watkins R, Plourde M Human apolipoprotein E allele and docosahexaenoic acid intake modulate peripheral cholesterol homeostasis in mice. J Nutr Biochem. 2016-08-01 [PMID: 27239755] (WB, Mouse)
• Rideout TC, Carrier B, Wen S et al. Complementary Cholesterol-Lowering Response of a Phytosterol/alpha-Lipoic Acid Combination in Obese Zucker Rats J Diet Suppl. 2015-02-09 [PMID: 25664679] (WB, Rat)
• Krueger WH, Tanasijevic B, Barber V et al. Cholesterol-Secreting and Statin-Responsive Hepatocytes from Human ES and iPS Cells to Model Hepatic Involvement in Cardiovascular Health. PLoS One 2013-07-11 [PMID: 23874411] (ICC/IF, Human)
• Wen S, Jadhav KS, Williamson DL, Rideout TC. Treadmill Exercise Training Modulates Hepatic Cholesterol Metabolism and Circulating PCSK9 Concentration in High-Fat-Fed Mice. J Lipids 2013-01-01 [PMID: 23862065] (WB, Mouse)
• Kim WS, Li H, Ruberu K et al. Deletion of Abca7 Increases Cerebral Amyloid-beta Accumulation in the J20 Mouse Model of Alzheimer's Disease. J Neurosci. 2013-03-06 [PMID: 23467355]
• Garbarino J, Pan M, Chin HF et al. STARD4 knockdown in HepG2 cells disrupts cholesterol trafficking associated with the plasma membrane, ER, and ERC J Lipid Res 2012-12-01 [PMID: 23033213]
• Wei J, Ching LC, Zhao JF et al. Essential role of transient receptor potential vanilloid type 1 in evodiamine-mediated protection against atherosclerosis. Acta Physiol (Oxf) 2013-02-01 [PMID: 23025809] (WB, Mouse)
• Murtola TJ, Syvala H, Pennanen P et al. The Importance of LDL and Cholesterol Metabolism for Prostate Epithelial Cell Growth PLoS One 2012-01-01 [PMID: 22761797] (WB, Human)
• Basak JM, Verghese PB, Yoon H, Kim J, Holtzman DM. Low-density Lipoprotein Receptor Represents an Apolipoprotein E-independent Pathway of A Beta Uptake and Degradation by Astrocytes. J Biol Chem;287(17):13959-71. 2012-04-20 [PMID: 22383525] (WB, Mouse)
• Domenger D, Dea D, Theroux L et al. The MPTP neurotoxic lesion model of Parkinson’s disease activates the apolipoprotein E cascade in the mouse brain. Experimental Neurology. 2011-01-01 [PMID: 22155743]
• Kolmakova A, Wang J, Brogan R et al. Deficiency of scavenger receptor class B type I negatively affects progesterone secretion in human granulosa cells. Endocrinology 2010-11-01 [PMID: 20844007] (WB, Human)
• Bogan RL, Hennebold JD. The reverse cholesterol transport system as a potential mediator of luteolysis in the primate corpus luteum. Reproduction 2010-01-01 [PMID: 19776099]
• Wahrle SE, Jiang H, Parsadanian M et al. Overexpression of ABCA1 reduces amyloid deposition in the PDAPP mouse model of Alzheimer disease. J Clin Invest 2008-02-01 [PMID: 18202749] (Mouse)

Review for LDLR Antibody (NB110-57162) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Verified Customer WB Other 07/30/2009

Summary

• Application: Western Blot
• Sample Tested: cell lines (Hep2 HepG2), Sample Amount: 50ug
• Species: Other
• Lot: YD112703

Bioinformatics

• Gene Symbol: LDLR
• Entrez:
  • 3949(Human)
  • 16835(Mouse)
  • 300438(Rat)
• Uniprot:
  • P01130()