LDLR Antibody (5E7)

Western Blot: LDLR Antibody (5E7) [H00003949-M01] - Analysis of LDLR expression in transfected 293T cell line by LDLR monoclonal antibody (M01), clone 5E7.Lane 1: LDLR transfected lysate(94.6 KDa).Lane 2: Non-transfected lysate.

ELISA: LDLR Antibody (5E7) [H00003949-M01] - LDL R Antibody (5E7) [H00003949-M01] - Detection limit for recombinant GST tagged LDLR is approximately 0.3ng/ml as a capture antibody.

Product Discontinued

LDLR Antibody (5E7) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: LDLR (NP_000518, 105 a.a. ~ 205 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PPKTCSQDEFRCHDGKCISRQFVCDSDRDCLDGSDEASCPVLTCGPASFQCNSSTCIPQLWACDNDPDCEDGSDEWPQRCRGLYVFQGDSSPCSAFEFHCL
• Specificity: LDLR - low density lipoprotein receptor (familial hypercholesterolemia)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: LDLR
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:100-1:2000
• Application Notes: Antibody reactivity against recombinant protein on ELISA. GST alone used as a negative control. It has been used for WB.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for LDLR Antibody (5E7)

• FH
• FHC
• LDL R
• LDL receptor
• LDLCQ2
• LDLR
• low density lipoprotein receptor
• low-density lipoprotein receptor class A domain-containing protein 3
• low-density lipoprotein receptor

Background

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LDLR Antibody (H00003949-M01)(1)

Publication using H00003949-M01

• Ueno M, Wu B, Nakagawa T et al. The expression of LDL receptor in vessels with blood-brain barrier impairment in a stroke-prone hypertensive model. Histochem Cell Biol. 2010-05-11 [PMID: 20458492]

Bioinformatics

• Gene Symbol: LDLR
• Entrez:
  • 3949(Human)
• Uniprot:
  • P01130(Human)