LBP Antibody (749405) [Unconjugated]

Images

 
Recombinant Mouse LBP induces IL-8 secretion in the THP‑1 human acute monocytic leukemia cell line in the presence of 5 ng/mL LPS in a dose-dependent manner (orange line), as measured by the Human IL-8 Quantikine kit ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications B/N
Clone
749405
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

LBP Antibody (749405) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse LBP
Gly25-Val481 (Ser102Arg, Tyr284His)
Accession # Q61805
Specificity
Detects mouse LBP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LBP is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
LBP
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Neutralization 1-5 ug/mL
Publications
Read Publication using
MAB6635 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LBP Antibody (749405) [Unconjugated]

  • LBP
  • lipopolysaccharide binding protein
  • lipopolysaccharide-binding protein
  • LPS-binding protein
  • MGC22233

Background

LBP (Lipopolysaccharide binding protein) is a 58‑62 kDa, single-chain glycoprotein member of the BPI/LBP family, BPI/PLUNC/PSP superfamily of lipid-binding proteins (1-3). It is secreted by a number of mammalian cell types, including hepatocytes (4), gingival keratinocytes (5), intestinal Paneth cells (6), and type II Greater alveolar cells (7). LBP is considered to be a class 1 APR (acute phase reactant) that is induced upon exposure to both IL-1 and IL-6 (8). These two cytokines appear upon immune cell exposure to pathogenic microbes. Following its synthesis and release, LBP is known to interact with bacterial wall components, lipopolysaccharide/LPS/Lipid A from Gram- (Gm-) bacteria, and lipoteichoic acid/LTA from Gm+ bacteria (9-13). In the case of LPS, this interaction appears to occur both in the bacterial cell wall, and within the intercellular space, where LPS micelles naturally form following bacterial death and cell wall dissolution (14-17). LBP is posited to induce disassembly of LPS micelles, allowing for LPS binding to LBP, and a heparin-mediated transfer of LPS from LBP to membrane-bound CD14 on the surface of monocytes/macrophages (15, 18). This CD14:LPS complex activates a TLR4:MD2 membrane complex, resulting in the production of NO and TNF-alpha (19). TNF-alpha serves as a chemoattractant for PMNs, and an initiator of coagulation that helps to wall-off and localize microbial elements (16). Notably, increased concentrations of LBP are also associated with parasitic infections (Trypanosoma), and may contribute to the immune response towards parasites (20). In addition to the above, LBP is also reported to transfer LPS to lipoproteins, particularly HDL and LDL (19, 21-23). For LDL, this transfer appears to be inhibitory to monocyte activation; for HDL, the effect may be either stimulatory or inhibitory, depending upon the circumstances (19). Mouse LBP is synthesized as a 481 amino acids (aa) precursor that contains a 25 aa signal sequence and a 456 aa mature region (aa 26-481) (24). It contains an N‑terminal LPS binding region plus a likely C-terminal LPS transfer region (24, 25). Mature mouse LBP shares 68% and 88% aa identity with human and rat LBP, respectively (11, 25).

  1. Beamer, L.J. et al. (1998) Protein Sci. 7:906.
  2. Schroder, N.W.J. & R.R. Schumann (2005) J. Endotoxin Res. 11:237.
  3. Miyake, K. (2006) J. Endotoxin Res. 12:195.
  4. Grube, B.J. et al. (1994) J. Biol. Chem. 269:8477.
  5. Ren, L. et al. (2004) J. Periodont. Res. 39:242.
  6. Hansen, G.H. et al. (2009) Histochem. Cell Biol. 131:727.
  7. Dentener, M.A. et al. (2000) Am. J. Respir. Cell Mol. Biol. 23:146.
  8. Schumann, R.R. et al. (1996) Mol. Cell. Biol. 16:3490.
  9. Weber, J.R. et. al. (2003) Immunity 19:269.
  10. Schroder, N.W.J. et al. (2004) J. Immunol. 173:2683.
  11. Su, G.L. et al. (1994) J. Immunol. 153:743.
  12. Schroder, N.W.J. et al. (2003) J. Biol. Chem. 178:15587.
  13. Wright, S.D. et al. (1989) J. Exp. Med. 170:1231.
  14. Hallatschek, W. et al. (2004) Eur. J. Immunol. 34:1441.
  15. Schumann, R.R. & E. Latz (2000) Chem. Immunol. 74:42.
  16. Mannel, D.N. & B. Echtenacher (2000) Chem. Immunol. 74:141.
  17. Tsukamoto, H. et al. (2010) Int. Immunol. 22:271.
  18. Heinzelmann, M. & H. Bosshart (2005) J. Immunol. 174:2280.
  19. Gallay, P. et al. (1993) Infect. Immun. 61:378.
  20. Ngure, R.M. et al. (2009) Res. Vet. Sci. 86:394.
  21. Levels, J.H.M. et al. (2005) Infect. Immun. 73:2321.
  22. Hubacek, J.A. et al. (1997) Biochem. Biophys. Res. Commun. 236:427.
  23. Thompson, P.A. & R.L. Kitchens (2006) J. Immunol. 177:4880.
  24. Lengacher, S. et al. (1995-1996) J. Inflamm. 47:165.
  25. Schumann, R.R. et al. (1990) Science 249:1429.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LBP Antibody (MAB6635)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Flow Cytometry.


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Bioinformatics

Gene Symbol LBP
Uniprot