Latexin Antibody (OTI1E10) - Azide and BSA Free

Western Blot: Latexin Antibody (OTI1E10) - Azide and BSA Free [NBP2-72069] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LXN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LXN.

Immunohistochemistry: Latexin Antibody (OTI1E10) - Azide and BSA Free [NBP2-72069] - Staining of paraffin-embedded Human lymph node tissue using anti-LXN mouse monoclonal antibody.

Flow Cytometry: Latexin Antibody (OTI1E10) - Azide and BSA Free [NBP2-72069] - HEK293T cells transfected with either Latexin Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-LXN antibody , and then analyzed by flow cytometry.

Immunohistochemistry: Latexin Antibody (OTI1E10) - Azide and BSA Free [NBP2-72069] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LXN mouse monoclonal antibody.

Immunohistochemistry: Latexin Antibody (OTI1E10) - Azide and BSA Free [NBP2-72069] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LXN mouse monoclonal antibody.

Immunohistochemistry: Latexin Antibody (OTI1E10) - Azide and BSA Free [NBP2-72069] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-LXN mouse monoclonal antibody.

Immunohistochemistry: Latexin Antibody (OTI1E10) - Azide and BSA Free [NBP2-72069] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-LXN mouse monoclonal antibody.

Immunohistochemistry: Latexin Antibody (OTI1E10) - Azide and BSA Free [NBP2-72069] - Staining of paraffin-embedded Human endometrium tissue using anti-LXN mouse monoclonal antibody.

Immunohistochemistry: Latexin Antibody (OTI1E10) - Azide and BSA Free [NBP2-72069] - Staining of paraffin-embedded Human Kidney tissue using anti-LXN mouse monoclonal antibody.

Immunohistochemistry: Latexin Antibody (OTI1E10) - Azide and BSA Free [NBP2-72069] - Staining of paraffin-embedded Human lymphoma tissue using anti-LXN mouse monoclonal antibody.

• Reactivity: Hu, Mu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI1E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Latexin Antibody (OTI1E10) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Latexin Antibody (OTI1E10) - Azide and BSA Free (NBP2-01382) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human LXN(NP_064554) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LXN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 25.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Latexin Antibody (OTI1E10) - Azide and BSA Free

• ECI
• ECIMUM
• Endogenous carboxypeptidase inhibitor
• Latexin
• LXN
• TCI
• TCIProtein MUM
• Tissue carboxypeptidase inhibitor

Background

Latexin, a protein possessing inhibitory activity against rat carboxypeptidase A1 (CPA1) and CPA2, is expressed in a neuronal subset in the cerebral cortex and cells in other neural and non-neural tissues of rat. Although latexin also inhibits mast-cell CPA (MCCPA), the expression of latexin in rat mast cells has not previously been confirmed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LXN