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LAT Antibody (LAT.10-17) [DyLight 680]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, Flow-CS, IHC, IHC-P
Clone
LAT.10-17
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 680

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-00289AF350
Alexa Fluor 405
NBP2-00289AF405
Alexa Fluor 488
NBP2-00289AF488
Alexa Fluor 532
NBP2-00289AF532
Alexa Fluor 594
NBP2-00289AF594
Alexa Fluor 647
NBP2-00289AF647
Alexa Fluor 700
NBP2-00289AF700
Alexa Fluor 750
NBP2-00289AF750
Allophycocyanin
NBP2-00289APC
Allophycocyanin/Cy7
NBP2-00289APCCY7
Biotin
NBP2-00289B
DyLight 350
NBP2-00289UV
DyLight 405
NBP2-00289V
DyLight 488
NBP2-00289G
DyLight 550
NBP2-00289R
DyLight 594
NBP2-00289DL594
DyLight 650
NBP2-00289C
DyLight 755
NBP2-00289IR
FITC
NBP2-00289F
HRP
NBP2-00289H
Janelia Fluor 549
NBP2-00289JF549
Janelia Fluor 646
NBP2-00289JF646
PE
NBP2-00289PE
PE/Atto594
NBP2-00289PEATT594
PE/Cy5.5
NBP2-00289PECY55
PE/Cy7
NBP2-00289PECY7
PerCP
NBP2-00289PCP
Unconjugated
NBP2-00289-100ug
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LAT Antibody (LAT.10-17) [DyLight 680] Summary

Immunogen
The immunogen for this antibody is LAT.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
LAT
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Flow (Cell Surface)
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for LAT Antibody (LAT.10-17) [DyLight 680]

  • 36 kDa phospho-tyrosine adapter protein
  • LAT
  • LAT1,36 kDa phospho-tyrosine adaptor protein
  • linker for activation of T cells
  • linker for activation of T-cells family member 1
  • p36-38
  • pp36

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-44502
Immunocytochemistry/Immunofluorescence: ZAP70 Antibody (2F3.2) [NBP2-44502] - Immunofluorescence Analysis of PFA-fixed Jurkat cells labeled with ZAP70 Antibody (2F3.2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red).
Immunohistochemistry-Paraffin: ZAP70 Antibody (2F3.2) [NBP2-44502] - Human Tonsil stained with ZAP70 Monoclonal Antibody (2F3.2).
Flow Cytometry: ZAP70 Antibody (2F3.2) [NBP2-44502] - Flow Cytometric Analysis of PFA-fixed Jurkat cells. ZAP70 Antibody (2F3.2) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
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Applications: Simple Western, Flow, ICC/IF, IHC, IHC-P
NTAL Antibody
[Unconjugated]
AF4066
Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line, Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human NTAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4066) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NTAL at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
THP‑1 human acute monocytic leukemia cell line was stained with Sheep Anti-Human NTAL Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4066, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Species: Hu
Applications: WB, CyTOF-ready, ICFlow
SLP-76/LCP2 [p
Tyr145] Antibody (776 ...
MAB7474
Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Phospho-SLP‑76/LCP2 (Y145) Monoclonal Antibody (Catalog # MAB7474) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-SLP‑76/LCP2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Human SLP‑76/LCP2 (Y145) at approximately 76-80 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human Phospho-SLP‑76/LCP2 (Y145) Monoclonal Antibody (Catalog # MAB7474). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
SLP‑76/LCP2 phosphorylated at Y145 was detected in immersion fixed Jurkat human acute T cell leukemia cells treated with (upper panel) or without (lower panel) Pervanadate using Mouse Anti-Human Phospho-SLP‑76/LCP2 (Y145) Monoclonal Antibody (Catalog # MAB7474) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Species: Hu
Applications: WB, Simple Western, ICC
SLC7A8 Antibody
(OTI7D6)
NBP1-47989
Western Blot: SLC7A8 Antibody (7D6) [NBP1-47989] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC7A8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC7A8.
Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Carcinoma of bladder tissue.
Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-SLC7A8 mouse monoclonal antibody.
Species: Hu
Applications: WB, IHC, IHC-P
CD98 Antibody
(1C11.7E3)
NBP2-36491
Western Blot: CD98 Antibody (1C11.7E3) [NBP2-36491] - Western blot analysis of human CD98 in recombinant protein, A431, Jurkat, HeLa and HepG2 lysates at 2.0 ug/ml.
Immunohistochemistry-Paraffin: CD98 Antibody (1C11.7E3) [NBP2-36491] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human kidney using CD98 antibody (clone 1C11.7E3) at 1:300 dilution. The antibody generated a distinct/intense membranous staining with relatively weaker cytoplasmic signal, and the immunostaining was exclusively observed in tubular epithelial cells with no signal in the Bowman's capsules.
Flow (Cell Surface): CD98 Antibody (1C11.7E3) [NBP2-36491] - Flow cytometry analysis of human CD98 in hPBMC. Primary antibody was used at 2.5 ug / million cells. Goat anti-mouse IgG (PE) was used at 0.5 ug / million cells. The red shift line represents the primary antibody, the blue line represents the cells alone and the green line represents the isotype control.
Species: Hu
Applications: WB, Flow, IHC, IHC-P, Flow-CS
ERK2 Antibody
(191801) [Unconjugate
MAB1230
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, U937 human histiocytic lymphoma cell line,PC‑12 rat adrenal pheochromocytoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat ERK2 Monoclonal Antibody (Catalog # MAB1230) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ERK2 at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ERK2 was detected in immersion fixed 3T3‑L1 mouse embryonic fibroblast adipose-like cell line using Mouse Anti-Human/Mouse/Rat ERK2 Monoclonal Antibody (Catalog # MAB1230) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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GRAP2 Antibody
(475804)
MAB4640
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, A549 human lung carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human GRAP2 Monoclonal Antibody (Catalog # MAB4640) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GRAP2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(2 Reviews)
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SYK Antibody
NBP1-32945
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Immunocytochemistry/Immunofluorescence: SYK Antibody [NBP1-32945] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: SYK Antibody [NBP1-32945] - Paraffin-embedded rat spleen dilution: 1:500.
Species: Hu, Mu, Rt
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SLC7A5/LAT1
Antibody (BU53)
NBP2-50465
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IL-2 [Unconjugated]
202-IL
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Lck [p Tyr394]
Antibody (755103)
MAB7500
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Phospho-Lck (Y394) Monoclonal Antibody (Catalog # MAB7500) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-Lck (Y394) at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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GRB2 Antibody
AF3846
Western blot shows lysates of HEK293 human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, Balb/3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat GRB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3846) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GRB2 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GRB2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 10 µg/mL Human GRB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3846) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Src [p Tyr419]
Antibody [Unconjugate
AF2685
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Simple Western lane view shows lysates of MBA‑MB‑468 human breast cancer cell line untreated (-) or treated (+) with 50 µM Pervanadate (PV) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Src (Y419) at approximately 64 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Src phosphorylated at Y419 was detected in immersion fixed paraffin-embedded sections of human colon using Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Mouse CD3+ T Cell,
Small Column Enrichme ...
MTCC-10-NOV
Mouse CD3+ T Cell, Small Column Enrichment Kit
Vav-1 [p Tyr160]
Antibody (751411) [Un ...
MAB37861
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Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes, loaded at 0.2 mg/mL. A specific band was detected for Human Phospho-Vav‑1 (Y160) at approximately 116 kDa (as indicated) using 1 µg/mL of Mouse Anti-HumanPhospho-Vav‑1 (Y160) Monoclonal Antibody (Catalog # MAB37861). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Vav‑1 phosphorylated at Y160 was detected in immersion fixed Jurkat human acute T cell leukemia cell line treated with (upper panel) or without (lower panel) Pervanadate using Mouse Anti-Human Phospho-Vav‑1 (Y160) Monoclonal Antibody (Catalog # MAB37861) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
(1 Publication)
Species: Hu
Applications: WB, Simple Western, ICC
CD4 Antibody
NBP1-19371
Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.
Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.
(8 Reviews)
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Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
EphB2 Antibody
[Unconjugated]
AF467
Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line and MBA‑MB‑468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for EphB2 at approximately 139 and 146 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western blot shows 25 ng of Recombinant Human EphB2 Fc Chimera (Catalog # 5189-B2) and Recombinant Mouse EphB2 Fc Chimera (Catalog # 467-B2). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EphB2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
EphB2 was detected in immersion fixed MBA‑MB‑468 human breast cancer cell line using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(9 Reviews)
(34 Publications)
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
CTLA-4
[Unconjugated]
7268-CT
Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) inhibits IL-2 secretion by stimulated Jurkat human acute Tcell leukemia cells. The ED50 for this effect is 0.03-0.15 μg/mL whenstimulated with 1 μg/mL Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1).
(3 Reviews)
(1 Publication)
Species: Hu

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-00289AF350
Alexa Fluor 405 NBP2-00289AF405
Alexa Fluor 488 NBP2-00289AF488
Alexa Fluor 532 NBP2-00289AF532
Alexa Fluor 594 NBP2-00289AF594
Alexa Fluor 647 NBP2-00289AF647
Alexa Fluor 700 NBP2-00289AF700
Alexa Fluor 750 NBP2-00289AF750
Allophycocyanin NBP2-00289APC
Allophycocyanin/Cy7 NBP2-00289APCCY7
Biotin NBP2-00289B
DyLight 350 NBP2-00289UV
DyLight 405 NBP2-00289V
DyLight 488 NBP2-00289G
DyLight 550 NBP2-00289R
DyLight 594 NBP2-00289DL594
DyLight 650 NBP2-00289C
DyLight 680 NBP2-00289FR
DyLight 755 NBP2-00289IR
FITC NBP2-00289F
HRP NBP2-00289H
Janelia Fluor 549 NBP2-00289JF549
Janelia Fluor 646 NBP2-00289JF646
PE NBP2-00289PE
PE/Atto594 NBP2-00289PEATT594
PE/Cy5.5 NBP2-00289PECY55
PE/Cy7 NBP2-00289PECY7
PerCP NBP2-00289PCP
Learn about Custom Antibody Labeling

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Blogs on LAT.

Check out the latest blog posts on LAT.
CD3 (OKT3) as a marker of immune response efficiency
Our immune system is a powerful defense mechanism against infection, however different variables can cause our immune response to work for or against us.  CD3 (cluster of differentiation 3) is one component of our immune signal response that is co...  Read full blog post.
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Bioinformatics

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