Lass5 Antibody - BSA Free

Western Blot: Lass5 Antibody [NBP1-76964] - SK-N-SH lysate with LASS5 antibody at (A) 1 and (B) 2 ug/ml.

Western Blot: Lass5 Antibody [NBP1-76964] - Human neuroblastoma cell lines untreated or treated with various drugs. Image from verified customer review.

Immunohistochemistry: Lass5 Antibody - BSA Free [NBP1-76964] - Immunohistochemistry of Lass5 in mouse brain tissue with Lass5 antibody at 2.5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Lass5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Lass5 Antibody - BSA Free (NBP1-76964) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-Lass5 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of the human LASS5. The immunogen is located within the first 50 amino acids of LASS5. Amino Acid Squence: EGPADGYGYPRGRH
• Specificity: Multiple isoforms of LASS5 are known to exist. This antibody is predicted not to cross-react with the highly homologous LASS6.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CERS5
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 2.5 ug/mL
  • Immunohistochemistry-Paraffin
  • Western Blot 1-2 ug/ml
• Control Peptide:

  Lass5 Peptide (NBP1-76964PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Lass5 Antibody - BSA Free

• CerS5
• FLJ25304
• LAG1 homolog, ceramide synthase 5
• LAG1 longevity assurance homolog 5 (S. cerevisiae)
• LAG1 longevity assurance homolog 5
• MGC45411
• Trh4

Background

The LASS (longevity assurance homolog) family members represent a subgroup of the homeobox gene family and are highly conserved from yeasts to mammals. Six members of this family of proteins have been characterized (LASS1-6) and all are involved in ceramide synthesis during cell growth regulation and cancer differentiation. LASS5, also called Trh4, is a 392 amino acid endoplasmic reticulum, multi-pass membrane protein. Functioning as a dihydro-ceramide synthase, LASS5 is involved in the production of sphingolipids containing mainly one fatty acid donor (N-linked palmitoyl-ceramide) in a fumonisin B1-independent manner. It uses palmitoyl-CoA as an acyl donor and is involved in the synthesis of C14, C16 and C18-ceramide. LASS5 is the most abundantly expressed and predominant ceramide synthase isoform in lung epithelia. Recent studies show that LASS5 partially correct growth and apoptosis. Multiple isoforms of LASS5 are known to exist. This antibody is predicted not to cross-react with the highly homologous LASS6.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lass5 Antibody (NBP1-76964)(2)

Publications using NBP1-76964

• Huang Q, Kang T, Shen S et al. Extracellular vesicular delivery of ceramides from pulmonary macrophages to endothelial cells facilitates chronic obstructive pulmonary disease. Cell communication and signaling : CCS 2025-03-08 [PMID: 40055817]
• Zabielski P, Blachnio-Zabielska A, Lanza IR et al. Impact of insulin deprivation and treatment on sphingolipid distribution in different muscle subcellular compartments of streptozotocin-diabetic C57Bl/6 mice Am J Physiol Endocrinol Metab. 2013-12-23 [PMID: 24368672] (WB, Mouse)

Bioinformatics

• Gene Symbol: CERS5
• Uniprot:
  • NP_671723(Human)