LAMP-1/CD107a Antibody (1D4B)

Product Discontinued

LAMP-1/CD107a Antibody (1D4B) Summary

• Immunogen: Mouse embryo fobroblast tissue culture cell membranes. It has been mapped to the N-terminus of LAMP-1.
• Marker: Late Endosome Marker
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: LAMP1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry < = 0.25 ug/10^6 cells in 100 ul
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
  • Western Blot
• Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation (PMID: 3923938), immunohistochemical staining of acetone-fixed frozen sections (PMID: 3923938 & 3101702) , and immunofluorescence microscopy (PMID: 8647888). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 25832774). Use in Western blot reported in multiple pieces scientific literature
• Theoretical MW: 120 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for LAMP-1/CD107a Antibody (1D4B)

• CD107 antigen-like family member A
• CD107a antigen
• CD107a
• LAMP1
• LAMP-1
• LAMPA
• LGP120
• lysosomal-associated membrane protein 1
• lysosome-associated membrane glycoprotein 1
• Lysosome-associated membrane protein 1

Background

LAMP-1 (lysosome-associated membrane protein 1), also known as CD107a (cluster of differentiation 107a), is a major component of lysosomal membranes that plays an important role in lysosomal biogenesis, autophagy, and cholesterol metabolism (1). LAMP-1 is a type I transmembrane glycoprotein that is expressed on plasma membranes and the membranes of endosomes, autolysosomes, and lysosomes (1,2). Additionally, LAMP-1/CD107a is a commonly used marker for natural killer (NK) cell degranulation (3). LAMP-1 and another lysosomal-associated membrane protein, LAMP-2, together make up about half of all lysosome membrane proteins (1). Additionally, LAMP-1 has a role in presenting carbohydrate ligands to selectins (2). Human LAMP-1 protein is comprised of 417 amino acids (aa) with a theoretical molecular weight of 44.8 kDa; however, glycosylation can increase the molecular weight upwards of 120 kDa (1, 4). Structurally, LAMP-1 protein contains a large luminal/extracellular domain (29-382 aa), a helical transmembrane domain (383-405 aa), and a short cytoplasmic tail (406-417 aa) (1,2). Additionally, the protein has many N- and O-linked glycosylation sites which helps with stability in the membrane (1,2).

LAMP-1 plays an important role in autophagy-mediated ATP-release during apoptosis where lysosomes containing intracellular ATP migrate to the plasma membrane and, during exocytosis, LAMP-1 is exposed to the cell surface (5). Studies have found that knockdown of LAMP-1 blocks the ATP release from the cell (5). Furthermore, an absence of LAMP-1 and LAMP-2 leads to an accumulation of lysosomal cholesterol (6). Lysosomal membrane dysfunction or defects has also been associated with disease development (6,7). For example, one feature of pancreatitis is autophagy impairment which is caused by lysosomal dysfunction and a corresponding decrease in lysosomal-membrane associated proteins LAMP-1 and LAMP-2 (7).

References

1. Eskelinen E. L. (2006). Roles of LAMP-1 and LAMP-2 in lysosome biogenesis and autophagy. Molecular aspects of medicine, 27(5-6), 495-502. https://doi.org/10.1016/j.mam.2006.08.005

2. Cheng, X. T., Xie, Y. X., Zhou, B., Huang, N., Farfel-Becker, T., & Sheng, Z. H. (2018). Revisiting LAMP1 as a marker for degradative autophagy-lysosomal organelles in the nervous system. Autophagy, 14(8), 1472-1474. https://doi.org/10.1080/15548627.2018.1482147

3. Krzewski, K., & Coligan, J. E. (2012). Human NK cell lytic granules and regulation of their exocytosis. Frontiers in immunology, 3, 335. https://doi.org/10.3389/fimmu.2012.00335

4. Uniprot (P11279)

5. Wang, Y., Martins, I., Ma, Y., Kepp, O., Galluzzi, L., & Kroemer, G. (2013). Autophagy-dependent ATP release from dying cells via lysosomal exocytosis. Autophagy, 9(10), 1624-1625. https://doi.org/10.4161/auto.25873

6. Schwake, M., Schr0der, B., & Saftig, P. (2013). Lysosomal membrane proteins and their central role in physiology. Traffic (Copenhagen, Denmark), 14(7), 739-748. https://doi.org/10.1111/tra.12056

7. Gukovsky, I., Pandol, S. J., Mareninova, O. A., Shalbueva, N., Jia, W., & Gukovskaya, A. S. (2012). Impaired autophagy and organellar dysfunction in pancreatitis. Journal of gastroenterology and hepatology, 27 Suppl 2(Suppl 2), 27-32. https://doi.org/10.1111/j.1440-1746.2011.07004.x

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LAMP-1/CD107a Antibody (NB100-77683)(5)

Publications using NB100-77683

• Shin H, Park D, Kim J et al. Peripubertal requirement of Tsg101 in maintaining the integrity of membranous structures in mouse oocytes Cell proliferation 2022-06-29 [PMID: 35768997] (ICC/IF, Mouse)
• Alza NP, Conde MA, Scodelaro-Bilbao PG, Salvador GA Neutral lipids as early biomarkers of cellular fate: the case of alpha-synuclein overexpression Cell death & disease 2021-01-07 [PMID: 33414430]
• Li G, Li CX, Xia M et al. Enhanced Epithelial-to-Mesenchymal Transition Associated with Lysosome Dysfunction in Podocytes: Role of p62/Sequestosome 1 as a Signaling Hub. Cell. Physiol. Biochem. 2015-04-02 [PMID: 25832774] (ICC/IF, WB, Mouse)
• Gorbunov NV, McDaniel DP, Zhai M et al. Autophagy and mitochondrial remodelling in mouse mesenchymal stromal cells challenged with Staphylococcus epidermidis J. Cell. Mol. Med. 2015-02-26 [PMID: 25721260] (WB, Mouse)
  
  Details:
  LAMP-1/CD107a antibody (clone 1D4B) was used at 1ug/ml concentration in WB for analysis of lysates from bone marrow mesenchymal stromal cells /BMSCs challenged or not with Staphylococcus epidermidis in vitro (Figure 2).
• Xu M, Li XX, Chen Y et al. Enhancement of dynein-mediated autophagosome trafficking and autophagy maturation by ROS in mouse coronary arterial myocytes J. Cell. Mol. Med. 2014-06-09 [PMID: 24912985] (WB, Mouse)
  
  Details:
  Antibody used for WB in murine coronary arterial myocytes (CAMs) at 1:1000 dilution (Figure 4E).

Bioinformatics

• Gene Symbol: LAMP1
• Entrez:
  • 16783(Mouse)
• Uniprot:
  • P11438()