Laminin alpha 3 Antibody (CL3112) - Azide and BSA Free

Analysis in human stomach and lymph node tissues using NBP3-44416 antibody. Corresponding Laminin alpha 3 RNA-seq data are presented for the same tissues.

Staining of human colon shows moderate positivity in basement membrane of glandular cells.

Staining of human oral mucosa shows moderate positivity in basement membrane of squamous epithelial cells.

Staining of human lymph node shows no positivity in lymphoid cells as expected.

Staining of human stomach shows moderate positivity in basement membrane of glandular cells.

Analysis of purified human recombinant Laminin-332, Laminin-421, Laminin-511, Laminin-121 and Laminin-221.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL3112
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Laminin alpha 3 Antibody (CL3112) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Laminin alpha 3 Antibody (CL3112) - Azide and BSA Free (NBP2-42390) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of Q16787, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAMA3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Laminin alpha 3 Antibody (CL3112) - Azide and BSA Free

• BM600 150kD subunit
• BM600
• BM600-150kDa
• E170
• Epiligrin 170 kDa subunit
• epiligrin alpha 3 subunit
• Epiligrin subunit alpha
• epiligrin
• kalinin 165kD subunit
• Kalinin subunit alpha
• kalinin-165kDa
• lama3a
• laminin subunit alpha-3
• laminin, alpha 3 (nicein (150kD), kalinin (165kD), BM600 (150kD), epilegrin)
• laminin, alpha 3
• laminin-5 alpha 3 chain
• Laminin-5 subunit alpha
• Laminin-6 subunit alpha
• Laminin-7 subunit alpha
• LAMNA
• LOCS
• nicein 150kD subunit
• Nicein subunit alpha
• nicein-150kDa

Background

Laminins are basement membrane components thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. The protein encoded by this gene is the alpha-3 subunit of laminin 5, which is a complex glycoprotein composed of three subunits (alpha, beta, and gamma). Laminin 5 is thought to be involved in cell adhesion, signal transduction and differentiation of keratinocytes. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LAMA3