Laminin alpha 2 Antibody (CL3450) - Azide and BSA Free

Analysis in human heart muscle and tonsil tissues using NBP3-43794 antibody. Corresponding Laminin alpha 2 RNA-seq data are presented for the same tissues.

Staining of human heart muscle shows strong membranous positivity in cardiomyocytes.

Staining of human cerebral cortex shows moderate positivity in blood vessels.

Staining of human placenta shows moderate positivity in basement membrane of trophoblastic cells.

Staining of human tonsil shows no positivity in lymphoid cells as expected.

Staining of rat stomach shows strong positivity in basement membrane of glandular cells.

Analysis of purified human recombinant Laminin-211, Laminin-221, Laminin-332, Laminin-421, Laminin-511 and Laminin-121.

Staining of mouse heart muscle shows strong membranous positivity in cardiomyocytes.

Staining of rat heart muscle shows strong membranous positivity in cardiomyocytes.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: CL3450
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Laminin alpha 2 Antibody (CL3450) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Laminin alpha 2 Antibody (CL3450) - Azide and BSA Free (NBP2-46624) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P24043, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAMA2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Laminin alpha 2 Antibody (CL3450) - Azide and BSA Free

• LAMA2
• Laminin alpha 2
• Laminin M chain
• laminin subunit alpha-2
• laminin, alpha 2
• Laminin-12 subunit alpha
• Laminin-2 subunit alpha
• LAMM
• LAMMLaminin-4 subunit alpha
• Merosin heavy chain
• Merosin

Background

Laminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LAMA2