FKTN Antibody (7J5N6)

Western Blot: FKTN Antibody (7J5N6) [NBP3-15472] - Western blot analysis of extracts of various cell lines, using FKTN antibody (NBP3-15472) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunohistochemistry-Paraffin: FKTN Antibody (7J5N6) [NBP3-15472] - Immunohistochemistry of paraffin-embedded human colon using FKTN Rabbit mAb (NBP3-15472) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IHC-P
• Clone: 7J5N6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

FKTN Antibody (7J5N6) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 362-461 of human FKTN (O75072).
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FKTN
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.05% BSA, 50% glycerol, pH7.3
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for FKTN Antibody (7J5N6)

• CMD1X
• EC 2.-
• FCMDMGC134945
• fukutin
• Fukuyama type congenital muscular dystrophy (fukutin)
• Fukuyama type congenital muscular dystrophy protein
• Fukuyama-type congenital muscular dystrophy protein
• LGMD2MMGC134944
• MDDGA4
• MDDGB4
• MDDGC4
• MGC126857
• MGC138243

Background

FKTN genes encode fukutin proteins that in isoform 1 are 461 amino acids long at 54 kDA and at isoform 2 are 430 amino acids long at 49 kDA. These proteins are thought to participate in glycosylation of alpha-dystroglycan/DAG1 as it is probably a glycosyltransferase. Additionally, it may reinforce large a complex surrounding the outside and inside of muscle membranes, further enhancing brain development. FKTN is involved with the CNBP gene. Defects in this gene cause myscular dystrophy-dystroglycanopathy congenital types A4, B4, C4, and cadiomyopathy dilated type 1X. FKTN is also linked to optic atrophy, cleft lip, walker-warburg syndrome, dubowitz syndrome, retinal detachment, and neuronal migration disorders.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FKTN