Laminin alpha 1 Antibody (AL-4) [Unconjugated]

Laminin alpha 1 was detected in immersion fixed frozen sections of embryonic mouse decidua (E10.5) using 10 µg/mL Rat Anti-Mouse Laminin alpha 1 Monoclonal Antibody (Catalog # MAB4656) overnight at4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Laminin alpha 1 was detected in perfusion fixed frozen sections of mouse kidney glomeruli (panel A), skin (panel B), intestine (panel C), and skeletal muscle (panel D) using Rat Anti-Mouse Laminin alpha 1 Monoclonal Antibody (Catalog # MAB4656) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clone: AL-4
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Laminin alpha 1 Antibody (AL-4) [Unconjugated] Summary

• Immunogen: Purified fragment of chymotrypsin-digested mouse EHS tumor-derived Laminin-1
• Specificity: Detects the Hep-1 heparin binding domain of mouse Laminin A/alpha 1 subunit (1).
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Lama1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Laminin alpha 1 Antibody (AL-4) [Unconjugated]

• LAMA
• LAMA1 laminin, alpha 1
• LAMA1
• Laminin alpha 1
• S-LAM-alpha

Background

Laminins are heterotrimeric, non-collagenous glycoproteins composed of alpha, beta, and gamma chains. Through interactions with integrins, dystroglycan, and other receptors, Laminins contribute to cell differentation, cell shape and migration, maintenance of tissue phenotypes, and survival. Laminin-1 is comprised of alpha 1, beta 1, and gamma 1 subunits.

1. Skubitz, A.P. et al. (1988) J. Biol. Chem. 263:4861.
2. Skubitz, A.P. et al. (1987) Exp. Cell Res. 173:349.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Laminin alpha 1 Antibody (MAB4656)(9)

Publications using MAB4656

• Schevenels, G;Cabochette, P;America, M;Vandenborne, A;De Grande, L;Guenther, S;He, L;Dieu, M;Christou, B;Vermeersch, M;Germano, RFV;Perez-Morga, D;Renard, P;Martin, M;Vanlandewijck, M;Betsholtz, C;Vanhollebeke, B; A brain-specific angiogenic mechanism enabled by tip cell specialization Nature 2024-04-03 [PMID: 38570687] (Immunohistochemistry, Western Blot, Mouse, Zebrafish)
• Cruz, LC;Habibovic, A;Dempsey, B;Massafera, MP;Janssen-Heininger, YMW;Lin, MJ;Hoffman, ET;Weiss, DJ;Huang, SK;van der Vliet, A;Meotti, FC; Identification of tyrosine brominated extracellular matrix proteins in normal and fibrotic lung tissues Redox biology 2024-05-01 [PMID: 38430684] (Immunohistochemistry, Mouse)
• Anthony A. Stephenson, Stefan Nicolau, Tatyana A. Vetter, Gabrielle P. Dufresne, Emma C. Frair, Jessica E. Sarff et al. CRISPR-Cas9 homology-independent targeted integration of exons 1–19 restores full-length dystrophin in mice Molecular Therapy - Methods & Clinical Development 2023-09-14 [PMID: 37706184] (Immunohistochemistry, Mouse)
• Q Liu, JH Lee, HM Kang, CH Kim Identification of the niche and mobilization mechanism for tissue-protective multipotential bone marrow ILC progenitors Science Advances, 2022-11-23;8(47):eabq1551. 2022-11-23 [PMID: 36417511] (IHC, Transgenic Mouse)
• Nicolas Wein, Tatyana A. Vetter, Adeline Vulin, Tabatha R. Simmons, Emma C. Frair, Adrienne J. Bradley et al. Systemic delivery of an AAV9 exon-skipping vector significantly improves or prevents features of Duchenne muscular dystrophy in the Dup2 mouse Molecular Therapy - Methods & Clinical Development 2022-09-08 [PMID: 35949298]
• Tabatha R. Simmons, Tatyana A. Vetter, Nianyuan Huang, Adeline Vulin-Chaffiol, Nicolas Wein, Kevin M. Flanigan Pre-clinical dose-escalation studies establish a therapeutic range for U7snRNA-mediated DMD exon 2 skipping Molecular Therapy - Methods & Clinical Development 2021-06-11 [PMID: 33898631]
• Emanuela Pasciuto, Oliver T. Burton, Carlos P. Roca, Vasiliki Lagou, Wenson D. Rajan, Tom Theys et al. Microglia Require CD4 T Cells to Complete the Fetal-to-Adult Transition Cell 2020-08-06 [PMID: 32702313] (Immunohistochemistry, Mouse)
• Dina B. AbuSamra, Jérôme Mauris, Pablo Argüeso Galectin-3 initiates epithelial-stromal paracrine signaling to shape the proteolytic microenvironment during corneal repair Science Signaling 2019-07-18 [PMID: 31311846] (Immunohistochemistry, Mouse)
• A Perrin, J Rousseau, JP Tremblay Increased Expression of Laminin Subunit Alpha 1 Chain by dCas9-VP160 Mol Ther Nucleic Acids, 2016-12-10;6(0):68-79. 2016-12-10 [PMID: 28325301] (ICC, IHC, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: Lama1