Laminin alpha 1 Antibody (775016) [Unconjugated]

Images

 
U2OS human osteosarcoma cell line was stained with Mouse Anti-Human Laminin alpha 1 Monoclonal Antibody (Catalog # MAB4187, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed ...read more
Laminin alpha 1 was detected in immersion fixed U2OS human osteosarcoma cell line (positive stain) and A549 human lung carcinoma cell line (negative stain) using Mouse Anti-Human Laminin alpha 1 Monoclonal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications CyTOF-ready, Flow, ICC/IF
Clone
775016
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Laminin alpha 1 Antibody (775016) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Laminin alpha 1
Leu22-Met269
Accession # P25391
Specificity
Detects human Laminin alpha 1 in direct ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Laminin alpha 1 Antibody (775016) [Unconjugated]

  • LAMA
  • LAMA1 laminin, alpha 1
  • LAMA1
  • Laminin alpha 1
  • S-LAM-alpha

Background

Laminin subunit alpha 1 (LAMA1) is a secreted 400 kDa extracellular matrix glycoprotein that contributes to the formation of basement membrane Laminin isoforms 1 and 3. It is one of three subunits ( alpha, beta, and gamma ) that interact via their coiled-coil domains to form the approximately 800 kDa cruciform, disulfide-linked, Laminin heterotrimer. The 3058 amino acid (aa) residue mature human alpha 1 chain contains an N-terminal Laminin VI domain (aa 18‑269), followed by domains V through III containing 17 EGF-like repeats, the coiled-coil domains II and I, and five globular, Laminin G-like domains. Over aa 22‑269, human Laminin alpha 1 shares 95% and 91% aa sequence identity with canine and mouse alpha 1 chain, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 IC4187U
Alexa Fluor 405 IC4187V
Alexa Fluor 488 IC4187G
Alexa Fluor 532 FAB4187X
Alexa Fluor 594 IC4187T
Alexa Fluor 647 IC4187R
Alexa Fluor 700 IC4187N
Alexa Fluor 750 IC4187S
Allophycocyanin FAB4187A
Biotin FAB4187B
DyLight 350 FAB4187D
DyLight 405 FAB4187E
DyLight 488 FAB4187K
DyLight 550 FAB4187L
DyLight 594 FAB4187M
DyLight 650 FAB4187W
DyLight 680 FAB4187Y
DyLight 755 FAB4187Z
FITC FAB4187F
Janelia Fluor 549 FAB4187I
Janelia Fluor 646 FAB4187J
PE FAB4187P
PerCP FAB4187C

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