Laminin-1 Antibody (AL-3) [Unconjugated]

Laminin‑1 was detected in immersion fixed frozen sections of embryonic mouse parietal endoderm (E10.5) using Rat Anti-Mouse Laminin‑1 Monoclonal Antibody (Catalog # MAB25491) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clone: AL-3
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Laminin-1 Antibody (AL-3) [Unconjugated] Summary

• Immunogen: Purified fragment of chymotrypsin-digested mouse EHS tumor-derived Laminin-1
• Specificity: Detects mouse Laminin‑1 in Western blots. This antibody reacts with mouse Laminin-1 beta chain (1).
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Laminin-1 Antibody (AL-3) [Unconjugated]

• LAMA
• LAMA1
• laminin subunit alpha 1
• Laminin1
• Laminin-1
• Laminin-111
• PTBHS
• S-LAM-alpha

Background

Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with integrins, dystroglycan, and other receptors, laminins contribute to cell differentation, cell shape and migration, maintenance of tissue phenotypes, and survival (1, 2).

1. Skubitz, A.P. et al. (1988) J Biol Chem. 263:4861
2. Skubitz, A.P. et al. (1987) Exp. Cell Res. 173:349.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.