Ku70/XRCC6 Antibody (KU729) [Alexa Fluor™ Plus 555]

• Reactivity: Hu, Pm, Ch(-), Mu(-), Rt(-)
• Applications: Flow, ICC/IF
• Clone: KU729
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 555

Ku70/XRCC6 Antibody (KU729) [Alexa Fluor™ Plus 555] Summary

• Immunogen: Nuclear extract of human HL-60 cells
• Localization: Nuclear
• Marker: Nuclear Marker
• Specificity: Recognizes a dimer of two proteins of 70kDa and ~80kDa, identified as two subunits of Ku. This monoclonal antibody recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: XRCC6
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Does not react with Mouse, Rat and Chicken.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Ku70/XRCC6 Antibody (KU729) [Alexa Fluor™ Plus 555]

• 5'-deoxyribose-5-phosphate lyase Ku70
• 5'-dRP lyase Ku70
• 70 kDa subunit of Ku antigen
• ATP-dependent DNA helicase 2 subunit 1
• ATP-dependent DNA helicase II 70 kDa subunit
• CTC box binding factor 75 kDa subunit
• CTC box-binding factor 75 kDa subunit
• CTC75
• CTCBF
• D22S731
• EC 3.6.4.-
• EC 4.2.99.-
• G22P1
• G22P1Ku70
• Ku autoantigen p70 subunit
• Ku autoantigen, 70kDa
• Ku70
• ML8
• ML8,70 kDa subunit
• thyroid-lupus autoantigen p70
• Thyroid-lupus autoantigen
• TLAA
• TLAAD22S671
• X-ray repair complementing defective repair in Chinese hamster cells 6DNA repair protein XRCC6
• X-ray repair cross-complementing protein 6
• XRCC6

Background

The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins. The p70/p80 dimer is important for function of a 460kDa DNAdependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: XRCC6