Kremen-2 Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Kremen-2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Kremen-2
  Gln19-Ala364
  Accession # Q8NCW0
• Specificity: Detects human Kremen-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross‑reactivity with recombinant mouse Kremen-2 and recombinant rat Kremen-2 is observed and less than 15% cross-reactivity with recombinant human Kremen-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KREMEN2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kremen-2 Antibody [Unconjugated]

• Dickkopf receptor 2
• kremen protein 2
• Kremen2
• Kremen-2
• kringle containing transmembrane protein 2
• Kringle domain-containing transmembrane protein 2
• KRM2Kringle-containing protein marking the eye and the nose
• MGC10791
• MGC16709

Background

Kremen (Kringle-containing protein marking the eye and the nose) proteins are type I transmembrane proteins that contain extracellular kringle, WSC and CUB domains and an intracellular region without any conserved motifs (1). Two related members, Kremen-1 and -2, have been identified. Kremens bind a subset of the secreted Dickkopf (Dkk) proteins (Dkk-1, -2, and -4) with high affinity to modulate the canonical Wnt signaling pathway that is transduced by the ternary receptor complex composed of Wnt, the seven-transmembrane domain receptor Frizzled, and the LDL-receptor-related protein 5/6 (LRP5/6) coreceptor (2, 3). Within the Dkk family, Dkk-1 and -4 bind directly to the LRP5/6 coreceptor to antagonize the canonical Wnt/ beta -catenin signaling pathway, but not the planar cell polarity (PCP) signaling pathway that does not involve LRP5/6 (4). In contrast, Dkk-3 has no effect on Wnt signaling and Dkk-2 can function either as an LRP agonist or antagonist, depending on whether the cell expresses Kremen (5). Kremen cooperates with Dkk to antagonize Wnt signaling via formation of a Kremen-Dkk-LRP ternary complex that triggers the internalization and clearance of the complex from the cell surface (3). All three extracellular domains but not the cytoplasmic region of a membrane anchored Kremen are needed for binding to the second cysteine-rich domain of Dkks (3). Human Kremen-2 cDNA encodes a 462 amino acid (aa) glycosylated protein with a putative 18 aa signal peptide, a 353 aa extracellular domain, a 17 aa transmembrane domain and a 75 aa cytoplasmic domain. In the extracellular domain, it shares 88% and 42% amino acid sequence identity with mouse Kremen-2 and human Kremen-1, respectively.

1. Nakamura, T. et al. (2001) Biochim. Biophys. Acta 1518:63.
2. Davidson G. et al. (2002) Development 129:5587.
3. Mao, B. et al. (2002) Nature 417:664.
4. Zorn, A.M. (2001) Curr. Biol. 11:R592.
5. Mao, B. and C. Niehrs (2003) Gene 302:179.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KREMEN2
• Uniprot:
  • Q8NCW0()