KPNA3 Antibody

Western Blot: KPNA3 Antibody [NB100-81650] - Association of NCBP3 and NCBP2 with NCBP1 and proteins involved in RNA processing. Western blot analysis of representative GFP-precipitated proteins identified by AP-MS analysis. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms9192), licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: KPNA3 Antibody [NB100-81650] - Human prostate carcinoma. Antibody used at a dilution of 1:200.

Western Blot: KPNA3 Antibody [NB100-81650] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-81650 used for WB at 0.4 mcg/ml (A and B) and used for IP at 3 mcg/mg lysate.

• Reactivity: Hu
• Applications: WB, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

KPNA3 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human karyopherin alpha 3 using the numbering given in entry NP_002258.2 (GeneID 3839).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KPNA3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  KPNA3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for KPNA3 Antibody

• hSRP1
• importin alpha 4
• Importin alpha Q2
• importin alpha-3
• importin subunit alpha-3
• importin-alpha-Q2
• IPOA4
• karyopherin alpha 3 (importin alpha 4)
• Karyopherin subunit alpha-3
• qip2
• SRP1
• SRP1gamma
• SRP1-gamma
• SRP4

Background

The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA3, encodes a protein similar to certain nuclear transport proteins of Xenopus and human. The predicted amino acid sequence shows similarity to Xenopus importin, yeast SRP1, and human RCH1 (KPNA2), respectively. The similarities among these proteins suggests that karyopherin alpha-3 may be involved in the nuclear transport system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KPNA3 Antibody (NB100-81650)(4)

Publications using NB100-81650

• Gebhardt A, Habjan M, Benda C et al. mRNA export through an additional cap-binding complex consisting of NCBP1 and NCBP3. Nat Commun 2015-09-18 [PMID: 26382858] (WB, Human)
• Zhang P, Yang X, Ma X et al. Antitumor effects of pharmacological EZH2 inhibition on malignant peripheral nerve sheath tumor through the miR-30a and KPNB1 pathway. Mol Cancer 2015-01-01 [PMID: 25890085] (IHC)
• Zhang P, Garnett J, Creighton CJ et al. EZH2-miR-30d-KPNB1 pathway regulates malignant peripheral nerve sheath tumour cell survival and tumourigenesis. J. Pathol. 2014-02-01 [PMID: 24132643] (IHC-P, WB, Human)
• Okazaki K, Nakayama N, Nariai Y et al. Nuclear localization signal in a cancer-related transcriptional regulator protein NAC1. Carcinogenesis 2012-10-01 [PMID: 22665369]

Bioinformatics

• Gene Symbol: KPNA3
• Entrez:
  • 3839(Human)
• Uniprot:
  • O00505(Human)