Importin alpha 3/KPNA4 Antibody

Images

 
Western Blot: Importin alpha 3/KPNA4 Antibody [NBP1-31260] - A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell ...read more
Immunocytochemistry/ Immunofluorescence: Importin alpha 3/KPNA4 Antibody [NBP1-31260] - Analysis of HeLa, using KPNA4 antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Importin alpha 3/KPNA4 Antibody [NBP1-31260] - Paraffin-embedded H1299 xenograft, using antibody at 1:500 dilution.
Western Blot: Importin alpha 3/KPNA4 Antibody [NBP1-31260] - Sample (30 ug of whole cell lysate) A: 293T B: HepG2 C: Molt-4 7.5% SDS PAGE diluted at 1:150
Western Blot: Importin alpha 3/KPNA4 Antibody [NBP1-31260] - A. 30 ug Rat2 whole cell lysate/extract 7.5 % SDS-PAGE KPNA4 antibody [C3], C-term dilution: 1:1000

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.73 mg/ml

Order Details

Importin alpha 3/KPNA4 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human KPNA4. The exact sequence is proprietary.
Localization
Cytoplasm, Nucleus
Predicted Species
Bovine (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KPNA4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-31260 in the following applications:

Publications
Read Publication using
NBP1-31260 in the following applications:

  • WB
    1 publication

Reactivity Notes

Chimpanzee (100%)

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.73 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Importin alpha 3/KPNA4 Antibody

  • Importin alpha 3
  • Importin alpha Q1
  • importin subunit alpha-4
  • importin-alpha-Q1
  • IPOA3
  • karyopherin alpha 4 (importin alpha 3)
  • KPNA4
  • MGC12217
  • MGC26703
  • Qip1
  • QIP12
  • QIP1Karyopherin subunit alpha-4
  • SRP3

Background

The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00003836-M01
Species: Hu, Rt
Applications: WB, ELISA, ICC/IF, IHC-P
NBP2-52501
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, IF
NB100-81650
Species: Hu
Applications: WB, IHC, IHC-P, IP, PLA
NB100-314
Species: Hu
Applications: WB, IP, PLA
25470002
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
PP-H4417-00
Species: Hu
Applications: WB, IP, DirELISA
NBP2-29323
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
NB600-225
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NBP2-15412
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP2-02665
Species: Hu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-01345
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-88650
Species: Hu
Applications: WB, IHC, IHC-P

Publications for Importin alpha 3/KPNA4 Antibody (NBP1-31260)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

Review for Importin alpha 3/KPNA4 Antibody (NBP1-31260) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-31260:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Importin alpha 3/KPNA4 NBP1-31260
Enlarge
reviewed by:
Anonymous
WB Human 08/31/2015
View

Summary

ApplicationWestern Blot
Sample TestedLNCaP whole cell lysate
SpeciesHuman
Lot39708

Blocking

Blocking Details5% skim milk in TBST buffer. Room temperature for 1 hour

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG, HRP
Secondary Manufacturer Cat#CST: 7074
Secondary Concentration1:1000 dilution

Details

Detection NotesA SuperSignal West Dura (Thermo scientific, Cat# 34076) kit and ChemiDoc

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Importin alpha 3/KPNA4 Antibody (NBP1-31260) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Importin alpha 3/KPNA4 Products

Bioinformatics Tool for Importin alpha 3/KPNA4 Antibody (NBP1-31260)

Discover related pathways, diseases and genes to Importin alpha 3/KPNA4 Antibody (NBP1-31260). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Importin alpha 3/KPNA4 Antibody (NBP1-31260)

Discover more about diseases related to Importin alpha 3/KPNA4 Antibody (NBP1-31260).
 

Pathways for Importin alpha 3/KPNA4 Antibody (NBP1-31260)

View related products by pathway.

PTMs for Importin alpha 3/KPNA4 Antibody (NBP1-31260)

Learn more about PTMs related to Importin alpha 3/KPNA4 Antibody (NBP1-31260).

Blogs on Importin alpha 3/KPNA4

There are no specific blogs for Importin alpha 3/KPNA4, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
08/31/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol KPNA4
Entrez
Uniprot