KNTC1 Antibody

Immunoprecipitation: KNTC1 Antibody [NB100-88131] - Detection of human ROD/KNTC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ROD/KNTC1 antibody NB100-88131 used for IP at 3 ug per reaction. ROD/KNTC1 was also immunoprecipitated by rabbit anti-ROD/KNTC1 antibody NB100-88130. For blotting immunoprecipitated ROD/KNTC1, NB100-88131 was used at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Immunoprecipitation: KNTC1 Antibody [NB100-88131] - Detection of human ROD/KNTC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ROD/KNTC1 antibody NB100-88131 used for IP at 3 ug per reaction. ROD/KNTC1 was also immunoprecipitated by rabbit anti-ROD/KNTC1 antibody NB100-88130. For blotting immunoprecipitated ROD/KNTC1, NB100-88131 was used at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

• Reactivity: Hu
• Applications: IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

KNTC1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 2159 and 2209 of human rough deal homolog using the numbering given in entry NP_055523.1 (GeneID 9735).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KNTC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 5 ug/mg lysate
• Application Notes: This antibody is not recommended for Western Blot analysis.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for KNTC1 Antibody

• FLJ36151
• hRod
• hsROD
• KIAA0166Rough Deal homolog, centromere/kinetochore protein
• kinetochore associated 1
• kinetochore-associated protein 1
• Rod
• RODHsROD
• Rough deal homolog

Background

This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. Experimental evidence indicated that the encoded protein functioned in a similar manner to that of the Drosophila rough deal protein. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KNTC1
• Entrez:
  • 9735(Human)
• Uniprot:
  • NP_055523.1(Human)