KMT1A/SUV39H1 Antibody

Images

 
Western Blot: KMT1A/SUV39H1 Antibody [NBP2-17086] - Non-transfected (-) and transfected (+) 293T whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SUV39H1 antibody [N3C3]. ...read more
Immunocytochemistry/ Immunofluorescence: KMT1A/SUV39H1 Antibody [NBP2-17086] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: KMT1A/SUV39H1 Antibody [NBP2-17086] - Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using antibody at 1:100 dilution.
Western Blot: KMT1A/SUV39H1 Antibody [NBP2-17086] - Sample (30 ug of whole cell lysate) A: H1299 B: Hep G2 C: Raji 10% SDS PAGE gel, diluted at 1:1000.
Western Blot: KMT1A/SUV39H1 Antibody [NBP2-17086] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.
Western Blot: KMT1A/SUV39H1 Antibody [NBP2-17086] - Western blot with anti Suv39H1 after siControl and siSUV39H1 in MCF7 cells. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, MkSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
     

Genetic Strategies

   

Order Details

KMT1A/SUV39H1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SUV39H1. The exact sequence is proprietary.
Localization
Nucleus; Centromere
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SUV39H1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-17086 in the following applications:

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for KMT1A/SUV39H1 Antibody

  • H3-K9-HMTase 1
  • Histone H3-K9 methyltransferase 1
  • histone-lysine N-methyltransferase SUV39H1
  • histone-lysine N-methyltransferase, H3 lysine-9 specific 1
  • KMT1AEC 2.1.1.43
  • Lysine N-methyltransferase 1A
  • MG44
  • Position-effect variegation 3-9 homolog
  • Su(var)3-9 homolog 1
  • suppressor of variegation 3-9 (Drosophila) homolog 1
  • suppressor of variegation 3-9 homolog 1 (Drosophila)
  • Suppressor of variegation 3-9 homolog 1
  • Suppressor of variegation 3-9 homolog 1, Suvar) 3-9 homolog
  • SUV39H

Background

This gene is a member of the suppressor of variegation 3-9 homolog family and encodes a protein with a chromodomain and a C-terminal SET domain. This nuclear protein moves to the centromeres during mitosis and functions as a histone methyltransferase, methylating Lys-9 of histone H3. Overall, it plays a vital role in heterochromatin organization, chromosome segregation, and mitotic progression. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for KMT1A/SUV39H1 Antibody (NBP2-17086) (0)

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Review for KMT1A/SUV39H1 Antibody (NBP2-17086) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-17086:
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Images Ratings Applications Species Date Details
Western Blot KMT1A/SUV39H1 NBP2-17086
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reviewed by:
Smita Singh
WB Human 07/01/2014
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Summary

ApplicationWestern Blot
Sample TestedWhole cell extract form MCF7 cells
SpeciesHuman
CommentsExposure time for the antibody was a little longer, probably because of less abundance of the protein in MCF-7 cells

Blocking

Blocking DetailsNon-fat dry milk (5%) in PBS

Primary Anitbody

Dilution Ratio1:1000 in PBS at RT for 3 hours

Secondary Antibody

Secondary DescriptionAnti rabbit IgG (HRP-conjugated)
Secondary Manufacturer Cat#ab6721 (Abcam)
Secondary Concentration1:20,000

Details

Detection NotesBio-Rad Immun-Star HRP Substrate was used with X-ray film, Exposure time: 1 min

Comments

CommentsExposure time for the antibody was a little longer, probably because of less abundance of the protein in MCF-7 cells

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for KMT1A/SUV39H1 Antibody (NBP2-17086) (0)

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Secondary Antibodies

 

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Recent Reviews

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1
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Smita Singh
07/01/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol SUV39H1
Uniprot