Klre-1 Antibody (854929) [Unconjugated]

Mouse splenocytes were stained with Rat Anti-Mouse NKp46/NCR1 PE‑conjugated Monoclonal Antibody (Catalog # FAB22252P) and either (A) Rat Anti-Mouse Klre‑1 Monoclonal Antibody (Catalog # MAB7099) or (B) Rat IgG2B Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 854929
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Klre-1 Antibody (854929) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line, CHO derived recombinant mouse Klre-1
  Lys94-Lys226
  Accession # NP_705818
• Specificity: Detects mouse Klre-1 in ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: Klre1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Klre-1 Antibody (854929) [Unconjugated]

• CD94
• Klre1
• Klre-1
• NKG2I

Background

Killer cell lectin-like receptor family E member 1 (Klre-1), also called NKG2I (NKG2 family member I), is a 24-28 kDa member of the NKG2/KLR family of proteins. It is expressed on mouse NK and NKT cells, and appears to serve as one component of two novel heterodimeric cell surface receptors. When complexed to KLRI1, Klre-1 inhibits NK cell cytotoxic activity. When complexed to KLRI2, Klre-1 activates NK cells, inducing IFN-gamma production and the activation of a cytolytic program. Although Klre-1 preferentially associates noncovalently with NLRI1 and I2, it apparently will form disulfide-linked homodimers in the absence of its heterodimer partners. Mouse Klre-1 is a 226 amino acid (aa) type II transmembrane protein. It contains an N-terminal cytoplasmic segment (aa 1-68) plus a 133 aa extracellular region (aa 94-226) that possesses one C-type lectin domain (aa 110-226). Over aa 94-226, mouse Klre-1 shares 82% aa identity with rat Klre-1. There does not appear to be a human structural ortholog to mouse Klre-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Klre1
• Uniprot:
  • NP_705818(Mouse)