Klotho beta Antibody (885935) [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Klotho beta Monoclonal Antibody (Catalog # MAB5889) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Klotho beta at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Klotho beta at approximately 177/170 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Klotho beta Monoclonal Antibody (Catalog # MAB5889) . This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clone: 885935
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Klotho beta Antibody (885935) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Klotho beta
  Phe53-Leu997
  Accession # Q86Z14
• Specificity: Detects human Klotho beta in direct ELISA and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: KLB
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Klotho beta Antibody (885935) [Unconjugated]

• betaKlotho
• beta-klotho
• BKL
• KLB
• klotho beta like
• Klotho beta
• klotho beta-like protein
• MGC142213

Background

Klotho beta (b-Klotho or KLB) is a 125-135 kDa member of the KL family, Glycosyl Hydrolase 1 superfamily of proteins.  It plays key role in the biology of the endocrine FGFs (FGF-15/19; 21; 23).  KLB appears to serve as a signaling cofactor for select FGFRs, including FGFR-1c, -2c, -3c and FGFR4.  The cofactor is known to constitutively interact with FGFRs, promote FGF binding to their appropriate FGFRs, and to bind directly to FGF-19 and FGF-21.  Notably, FGF-23 appears to utilize a-Klotho/KL for its FGFR signaling cofactor, and FGF-1, while not dependent upon KLB for signaling, can utilize KBL to potentiate certain activities.  KLB is expressed on select cells, including adipocytes and hepatocytes.  Human KLB is a 1044 amino acids (aa) type III transmembrane (TM) protein (i.e.-a type I TM protein lacking a signal sequence).  It contains a 996 aa extracellular region (aa 1-996) and a 27 aa cytoplasmic domain (aa 1018-1044).  The extracellular region contains two glycosyl hydrolase domains (aa 77-508 and 517-967) plus eleven potential N-linked glycosylation sites. Neither hydrolase domain is active as they lack a key Glu residue needed for activity.  Over aa 53-997, human KLB shares 80% aa sequence identity with mouse KLB.  And relative to KL/a-Klotho, human KLB and KL share just 44% aa sequence identity over the entire length of the molecules.     

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Klotho beta Antibody (MAB5889)(3)

Publications using MAB5889

• Z Tao, Y Cui, X Xu, T Han FGFR redundancy limits the efficacy of FGFR4-selective inhibitors in hepatocellular carcinoma Proceedings of the National Academy of Sciences of the United States of America, 2022-09-30;119(40):e2208844119. 2022-09-30 [PMID: 36179047] (Western Blot, Human)
• Stefano Bartesaghi, Kristina Wallenius, Daniel Hovdal, Mathias Liljeblad, Simonetta Wallin, Niek Dekker et al. Subcutaneous delivery of FGF21 mRNA therapy reverses obesity, insulin resistance, and hepatic steatosis in diet-induced obese mice Molecular Therapy - Nucleic Acids 6/14/2022 [PMID: 35592498]
• SY Shi, YW Lu, J Richardson, X Min, J Weiszmann, WG Richards, Z Wang, Z Zhang, J Zhang, Y Li A systematic dissection of sequence elements determining ?-Klotho and FGF interaction and signaling Sci Rep, 2018-07-23;8(1):11045. 2018-07-23 [PMID: 30038432] (ELISA Development (Detection))

Bioinformatics

• Gene Symbol: KLB
• Uniprot:
  • Q86Z14()