KLF8 Antibody

Western Blot: KLF8 Antibody [NB100-1050] - (2ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence..

• Reactivity: Hu, Po
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

KLF8 Antibody Summary

• Description: Novus Biologicals Goat KLF8 Antibody (NB100-1050) is a polyclonal antibody validated for use in WB and ELISA. Anti-KLF8 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence VDMDKLINNLEVQ-C corresponding to N-Terminus according to NP_009181.2, NP_001152768.1.
• Specificity: This antibody is expected to recognise isoform 1 (NP_009181.2) and isoform 2 (NP_001152768.1).
• Predicted Species: Porcine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KLF8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 50 kDa band observed in human kidney lysates is reported in scientific literature (PMID: 15557280).
• Control:

  Human Kidney Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Porcine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for KLF8 Antibody

• Basic krueppel-like factor 3
• basic kruppel-like factor 3
• BKLF3DKFZp686O08126
• DXS741
• Krueppel-like factor 8
• Kruppel-like factor 8
• Zinc finger protein 741
• ZNF741MGC138314

Background

Kruppel-like factor proteins all contain a characteristic C-terminal DNA-binding domain containing 3 Kruppel-like zinc fingers, and they all recognize CACCC and GC boxes present in promoters and enhancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KLF8 Antibody (NB100-1050)(2)

Publications using NB100-1050

• Ding Q, Grammer JR, Nelson MA et al. p27Kip1 and cyclin D1 are necessary for focal adhesion kinase regulation of cell cycle progression in glioblastoma cells propagated in vitro and in vivo in the scid mouse brain. J Biol Chem 2005-02-01 [PMID: 15557280]
• van Vliet J, Turner J, Crossley M. Human Kruppel-like factor 8: a CACCC-box binding protein that associates with CtBP and represses transcription. Nucleic Acids Res 2000-05-01 [PMID: 10756197]

Bioinformatics

• Gene Symbol: KLF8
• Entrez:
  • 11279(Human)
• Uniprot:
  • NP_009181(Human)