KLF7 Antibody - Azide and BSA Free

KLF7 Antibody [NBP3-05084] - Analysis of Mouse kidney, using KLF7 antibody at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

KLF7 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit KLF7 Antibody - Azide and BSA Free (NBP3-05084) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 51-230 of human KLF7 (NP_001257871.1). SETFGEDLDCFLHASPPPCIEESFRRLDPLLLPVEAAICEKSSAVDILLSRDKLLSETCLSLQPASSSLDSYTAVNQAQLNAVTSLTPPSSPELSRHLVKTSQTLSAVDGTVTLKLVAKKAALSSVKVRSLISAHGRDVSGVLHEAMSSRGTTGNTQVQSPSNATTATGVFPGLTILPST
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KLF7
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for KLF7 Antibody - Azide and BSA Free

• Krueppel-like factor 7
• Kruppel-like factor 7 (ubiquitous)
• ubiquitous Kruppel-like transcription factor
• UKLFUbiquitous krueppel-like factor

Background

KLF7 is a transcriptional activator. It binds in vitro to the CACCC motif of the beta-globin promoter and to the SP1 recognition sequence. It is ubiquitous and highly expressed at low levels in brain and spinal cord in the adult, and in kidney and brain in the embryo. The acidic N terminal part may favor interaction with the basic domain of transcription factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: KLF7