KLF5 Antibody [Alexa Fluor® 647]

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

KLF5 Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human KLF5
  Leu85-Ala177
  Accession # Q13887
• Specificity: Detects human and mouse KLF5 in Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human (rh) KLF4, rhKLF6, recombinant mouse (rm) KLF4 and rmKLF15 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Knockout Validated
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KLF5 Antibody [Alexa Fluor® 647]

• Basic transcription element-binding protein 2
• BTEB2
• BTE-binding protein 2
• CKLFGC-box-binding protein 2
• Colon krueppel-like factor
• colon kruppel-like factor
• GC box binding protein 2
• IKLFbasic transcription element binding protein 2
• Intestinal-enriched krueppel-like factor
• intestinal-enriched kruppel-like factor
• KLF5
• Krueppel-like factor 5
• Kruppel-like factor 5 (intestinal)
• Transcription factor BTEB2

Background

Human KLF5, also known as basic transcription element binding protein 2 (BTEB2), is a widely expressed 51 kDa (457 aa) member of the kruppel C2H2-type zinc finger protein family. KLF5 contains three C2H2-type zinc fingers at the carboxyl-terminus that preferentially bind to cis-DNA elements that are GC rich. It is a transcription activator that promotes cell proliferation and oncogenesis. Over the region used as immunogen, human, mouse and canine KLF5 share 95% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.