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KLF4 Overexpression Lysate (Native)

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Western Blot: KLF4 Overexpression Lysate (Adult Normal) [NBL1-12320] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KLF4.
Western Blot: KLF4 Overexpression Lysate (Adult Normal) [NBL1-12320] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KLF4.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

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KLF4 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC206691 and based on accession number NM_004235. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens Kruppel-like factor 4 (gut) (KLF4), mRNA.
Gene
KLF4

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
49.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for KLF4

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for KLF4 Overexpression Lysate (Native)

  • Epithelial zinc finger protein EZF
  • EZF
  • EZFendothelial Kruppel-like zinc finger protein
  • GKLFKrueppel-like factor 4
  • Gut-enriched krueppel-like factor
  • KLF4
  • Kruppel-like factor 4 (gut)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.
KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.
KLF4 opens the door for stem cell research
KLF4 (Kruppel-like factor 4, Epithelial zinc finger protein EZF) is a zinc finger transcription factor thought to be involved in developmental differentiation and proliferation. It is considered a pluripotency reprogramming factor (PRF) due to its abi...  Read full blog post.

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Bioinformatics

Gene Symbol KLF4
Entrez