KLF2 Antibody

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Western Blot: KLF2 Antibody [NB100-1051] - Staining of Human Heart (A) and Lung (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC-Fr, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

KLF2 Antibody Summary

Immunogen
Peptide with sequence ALSEPILPSFST-C corresponding to N-Terminus according to NP_057354.1.
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
KLF2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 37 kDa band observed in human heart and lung lysates (calculated MW of 37.4 kDa band according to NP_057354.1).
Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Brain and Mouse Heart for this particular batch.
Use in Immunocytochemistry/immunofluorescence and Immunohistochemistry-frozen reported in scientific literature (PMID 24516105).
Positive Control
KLF2 Lysate (NBL1-12319)
Heart Lysate (NB820-59217)
Publications
Read Publications using
NB100-1051 in the following applications:

Reactivity Notes

Predicted cross-reactivity based on sequence identity:Canine, Porcine, Bovine. Mouse reactivity reported in scientific literature (PMID: 24024790). Rat reactivity reported in scientific literature (PMID: 24516105).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for KLF2 Antibody

  • KLF2
  • Krueppel-like factor 2
  • Kruppel-like factor 2 (lung)
  • Kruppel-like factor LKLF
  • LKLF
  • LKLFLung krueppel-like factor
  • lung Kruppel-like zinc finger transcription factor

Background

KLF2 binds to the CACCC box in the beta-globin gene promoter and activates transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for KLF2 Antibody (NB100-1051)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for KLF2 Antibody (NB100-1051) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on KLF2.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

Nucleolin Antibodies: Knowing When it's Time to Split
Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein that is a major protein in actively dividing cells. The stability of nucleolin is heavily cell proliferation-dependent, as nucleolin antibody studies have shown that degraded forms are relativ...  Read full blog post.

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Bioinformatics

Gene Symbol KLF2
Entrez
Uniprot