KLF2 Antibody

Western Blot: KLF2 Antibody [NB100-1051] - Staining of Human Heart (A) and Lung (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: KLF2 Antibody [NB100-1051] - Expression of KLF2 in cells infected with AdGFP and AdKLF2-GFP (24h). Infected cells are green and the arrowhead points to nuclear localisation of KLF2; bar=10um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-14966-x), licensed under a CC-BY license.

Product Discontinued

KLF2 Antibody Summary

• Immunogen: Peptide with sequence ALSEPILPSFST-C corresponding to N-Terminus according to NP_057354.1.
• Localization: Nuclear
• Predicted Species: Mouse (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KLF2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: Use in IHC-P reported in scientific literature (PMID:35494227) WB: Approx. 37 kDa band observed in human heart and lung lysates (calculated MW of 37.4 kDa band according to NP_057354.1).
  Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Brain and Mouse Heart for this particular batch.
  Use in ICC/IF and IHC-Frozen reported in scientific literature (PMID 24516105)..
• Control:

  KLF2 Overexpression Lysate
  Human Heart Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24516105 & 24024790).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for KLF2 Antibody

• KLF2
• Krueppel-like factor 2
• Kruppel-like factor 2 (lung)
• Kruppel-like factor LKLF
• LKLF
• LKLFLung krueppel-like factor
• lung Kruppel-like zinc finger transcription factor

Background

KLF2 binds to the CACCC box in the beta-globin gene promoter and activates transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KLF2 Antibody (NB100-1051)(7)

Publications using NB100-1051

• RytkOnen K, Faux T, Mahmoudian M et al. Histone H3K4me3 breadth in hypoxia reveals endometrial core functions and stress adaptation linked to endometriosis iScience 2022-05-01 [PMID: 35494227] (IHC-P, Human)
• Kamel NM, El-Tanbouly DM, Abdallah DM, Sayed HM PAR1, a therapeutic target for remote lung injury associated with hind limb ischemia/reperfusion: ERK5/KLF2-dependent lung capillary barrier preservation Chemico-biological interactions 2022-01-11 [PMID: 35031271] (WB, Human)
• Han JH, Jang KW,Myung CS Garcinia cambogia attenuates adipogenesis by affecting CEBPB and SQSTM1/p62-mediated selective autophagic degradation of KLF3 through RPS6KA1 and STAT3 suppression Autophagy 2021-06-08 [PMID: 34101546]
• Sindi Ha, Russomanno G, Satta S et Al. Therapeutic potential of KLF2-induced exosomal microRNAs in pulmonary hypertension Nat Commun 2020-03-04 [PMID: 32132543] (ICC/IF, Human)
• Haack KK, Marcus NJ, Del Rio R et al. Simvastatin treatment attenuates increased respiratory variability and apnea/hypopnea index in rats with chronic heart failure. Hypertension. 2014-05-01 [PMID: 24516105] (ICC/IF, IHC-Fr, Rat)
• Kim YS, Kwon JS, Hong MH et al. Restoration of angiogenic capacity of diabetes-insulted mesenchymal stem cells by oxytocin. BMC Cell Biol. 2013-09-11 [PMID: 24024790] (WB, Rat)
• Anderson KP, Kern CB, Crable SC, Lingrel JB. Isolation of a gene encoding a functional zinc finger protein homologous toerythroid Kruppel-like factor: identification of a new multigene family. Mol Cell Biol 1995-11-01 [PMID: 7565748]

Bioinformatics

• Gene Symbol: KLF2
• Entrez:
  • 10365(Human)
• Uniprot:
  • NP_057354.(Human)