Human Kininogen 1 ELISA Kit (Colorimetric) Summary
| Description |
This is a competitive enzyme immunoassay for the quantitative measurement of human Kininogen 1 in plasma and serum. |
| Standard Curve Range |
0.063 to 4 ug/mL |
| Sensitivity |
50 ng/mL |
| Assay Type |
Competitive ELISA |
| Inter-Assay |
CV% < 7.30% |
| Intra-Assay |
CV% < 4.80% |
| Spike Recovery |
94% |
| Sample Volume |
25 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
KNG1 |
Applications/Dilutions
| Dilutions |
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| Application Notes |
Useful for quantification. |
| Publications |
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Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Chromogen Substrate (1x): A stabilized peroxidase chromogen substrate tetramethylbenzidine.
- Human Kininogen 1 Microplate: A 96-wells polystyrene microplate coated with a polyclonal antibody against human Kininogen 1.
- Human Kininogen 1 Standard: Human Kininogen 1 in a buffered protein base,lyophilized.
- MIX Diluent Concentrate (10x): A 10-fold concentrated buffered protein base.
- Sealing Tapes: Pressure-sensitive sealing tapes that can be cut to fit the format of the individual assay.
- SP Conjugate (100x): A 100-fold concentrate.
- Stop Solution (1x): A 0.5 N hydrochloric acid solution to stop the chromogen substrate reaction.
- Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human Kininogen 1 ELISA Kit (Colorimetric)
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Publications for Kininogen 1 ELISA Kit (KA1040)(3)
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for Kininogen 1 ELISA Kit (KA1040). (Showing 1 - 1 of 1 FAQs).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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