Ki67/MKI67 Antibody [Unconjugated]

Ki67/MKI67 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Sheep Anti-Mouse Ki67/MKI67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7649) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and nucleoli. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Mu
• Applications: ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Ki67/MKI67 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse Ki67/MKI67
  Asn3053-Ser3177
  Accession # NP_001074586
• Specificity: Detects mouse Ki67/MKI67 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human Ki67/MKI67 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: MKI67
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ki67/MKI67 Antibody [Unconjugated]

• antigen identified by monoclonal Ki-67
• Antigen Ki67
• antigen KI-67
• Ki67
• Ki-67
• KIA
• Marker Of Proliferation Ki-67
• MIB-
• MIB-1
• MKI67
• PPP1R105
• Proliferation Marker Protein Ki-67
• proliferation-related Ki-67 antigen
• Protein Phosphatase 1
• Regulatory Subunit 105
• TSG126

Background

MKI67 (also Ki67 and TSG126) is a 350-370 kDa nuclear protein that belongs to a molecular group comprised of mitotic chromosome-associated proteins. Ki67 was originally recognized as an antigen associated with the monoclonal Ki67 antibody raised against Hodgkin's lymphoma nuclear material. Ki67 is contextually expressed, being potentially found in all cells that are not in the Go phase of the cell cycle. Thus, MKI67 qualifies as a cell proliferation marker. Functionally, Ki67 is known to interact with 160 kDa Hklp2, a protein that promotes centrosome separation and spindle bipolarity. It also directly interacts with NIFK, and apparently binds to UBF, thus playing a role in rRNA synthesis. Mouse MKI67 is 3177 amino acids (aa) in length. It contains one FHA domain (aa 8-101), followed by sixteen 120 aa repeats (aa 993-2872). There are two potential isoform variants. One isoform shows a 19 aa substitution for 1120-3177, while a second isoform contains a deletion of aa 1169-1409. Over aa 3053-3177, mouse Ki67 shares 46% and 74% aa sequence identity with the human and rat orthologs to Ki67, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ki67/MKI67 Antibody (AF7649)(30)

Publications using AF7649

• Chen, J;Sobecki, M;Krzywinska, E;Thierry, K;Masmoudi, M;Nagarajan, S;Fan, Z;He, J;Ferapontova, I;Nelius, E;Seehusen, F;Gotthardt, D;Takeda, N;Sommer, L;Sexl, V;Münz, C;DeNardo, D;Hennino, A;Stockmann, C; Fibrolytic vaccination against ADAM12 reduces desmoplasia in preclinical pancreatic adenocarcinomas EMBO molecular medicine 2024-10-30 [PMID: 39478152] (Immunohistochemistry, Mouse)
• Chaube, B;Citrin, KM;Sahraei, M;Singh, AK;de Urturi, DS;Ding, W;Pierce, RW;Raaisa, R;Cardone, R;Kibbey, R;Fernández-Hernando, C;Suárez, Y; Suppression of angiopoietin-like 4 reprograms endothelial cell metabolism and inhibits angiogenesis Nature communications 2023-12-12 [PMID: 38086791] (ICC, Mouse)
• Liang, Y;Wang, B;Chen, Q;Fu, X;Jiang, C;Lin, Z;Zhuang, Q;Zeng, Y;Liu, X;Zhang, D; Systemic delivery of glycosylated-PEG-masked oncolytic virus enhances targeting of antitumor immuno-virotherapy and modulates T and NK cell infiltration Theranostics 2023-10-02 [PMID: 37908722] (Immunohistochemistry, Mouse)
• Crossley, JL;Ostashevskaya-Gohstand, S;Comazzetto, S;Hook, JS;Guo, L;Vishlaghi, N;Juan, C;Xu, L;Horswill, AR;Hoxhaj, G;Moreland, JG;Tower, RJ;Levi, B; Itaconate-producing neutrophils regulate local and systemic inflammation following trauma JCI insight 2023-09-14 [PMID: 37707952] (IHC, Mouse)
• Wu, SR;Butts, JC;Caudill, MS;Revelli, JP;Dhindsa, RS;Durham, MA;Zoghbi, HY; Atoh1 drives the heterogeneity of the pontine nuclei neurons and promotes their differentiation Science advances 2023-06-30 [PMID: 37390208] (IHC, Mouse)
• Liu, X;Weng, W;He, L;Zhou, B; Genetic recording of in vivo cell proliferation by ProTracer Nature protocols 2023-06-02 [PMID: 37268780] (IHC, Mouse)
• M Ishan, Z Wang, P Zhao, Y Yao, S Stice, L Wells, Y Mishina, HX Liu Taste papilla cell differentiation requires tongue mesenchyme via ALK3-BMP signaling to regulate the production of secretory proteins bioRxiv : the preprint server for biology, 2023-04-04;0(0):. 2023-04-04 [PMID: 37066397] (IHC, Mouse)
• L Provitera, A Tomaselli, G Raffaeli, S Crippa, C Arribas, I Amodeo, S Gulden, GS Amelio, V Cortesi, F Manzoni, G Cervellini, J Cerasani, C Menis, N Pesenti, M Tripodi, L Santi, M Maggioni, C Lonati, S Oldoni, F Algieri, F Garrido, ME Bernardo, F Mosca, G Cavallaro Human Bone Marrow-Derived Mesenchymal Stromal Cells Reduce the Severity of Experimental Necrotizing Enterocolitis in a Concentration-Dependent Manner Cells, 2023-02-27;12(5):. 2023-02-27 [PMID: 36899900] (IHC, Mouse)
• Y Wang, ER Zoneff, JW Thomas, N Hong, LL Tan, DJ McGillivra, AW Perriman, KCL Law, LH Thompson, N Moriarty, CL Parish, RJ Williams, CJ Jackson, DR Nisbet Hydrogel oxygen reservoirs increase functional integration of neural stem cell grafts by meeting metabolic demands Nature Communications, 2023-01-28;14(1):457. 2023-01-28 [PMID: 36709345] (IHC, Mouse)
• Tabitha R. F. Green, Sean M. Murphy, Maria P. Moreno-Montano, Etienne Audinat, Rachel K. Rowe Reactive morphology of dividing microglia following kainic acid administration Frontiers in Neuroscience 9/29/2022 [PMID: 36248637]
• Feng Gao, Changgong Li, Susan M Smith, Neil Peinado, Golenaz Kohbodi, Evelyn Tran et al. Decoding the IGF1 signaling gene regulatory network behind alveologenesis from a mouse model of bronchopulmonary dysplasia eLife 10/10/2022 [PMID: 36214448]
• Feng Gao, Changgong Li, Susan M Smith, Neil Peinado, Golenaz Kohbodi, Evelyn Tran et al. Decoding the IGF1 signaling gene regulatory network behind alveologenesis from a mouse model of bronchopulmonary dysplasia eLife 2022-10-10 [PMID: 36214448] (Immunohistochemistry, Mouse)
• ST Narla, JL Duara, DS Bushnell, M Nouraie, J Holden, K Pfister, PC Lucas, S Sims-Lucas, CM Bates Role of ERK signaling in bladder urothelium in response to cyclophosphamide injury Physiological Reports, 2022-07-01;10(14):. 2022-07-01 [PMID: 35854647] (IHC, Mouse)
• ST Narla, L Rice, D Ostrov, DS Bushnell, JL Duara, CM Bates Durability of and role of AKT in FGF7p urothelial protection against cyclophosphamide Physiological Reports, 2022-06-01;10(12):e15358. 2022-06-01 [PMID: 35748317] (IHC, Mouse)
• Vera Gaun, Jeffrey R. Martens, James E. Schwob Lifespan of mature olfactory sensory neurons varies with location in the mouse olfactory epithelium and age of the animal Journal of Comparative Neurology 8/1/2022 [PMID: 35434783]
• ST Narla, L Rice, D Ostrov, SG Swarts, DW Siemann, DS Bushnell, JG Holden, JL Duara, CM Bates FGF7 peptide (FGF7p) mimetic mitigates bladder urothelial injury from cyclophosphamide Physiological Reports, 2022-04-01;10(7):e15241. 2022-04-01 [PMID: 35388988] (IHC, Mouse)
• F Gao, C Li, S Danopoulos, D Al Alam, N Peinado, S Webster, Z Borok, GA Kohbodi, S Bellusci, P Minoo Hedgehog-responsive PDGFRa(+) fibroblasts maintain a unique pool of alveolar epithelial progenitor cells during alveologenesis Cell Reports, 2022-04-05;39(1):110608. 2022-04-05 [PMID: 35385750] (IHC, Mouse)
• J Wang, R Hao, T Jiang, X Guo, F Zhou, L Cao, F Gao, G Wang, J Wang, K Ning, C Zhong, X Chen, Y Huang, J Xu, S Gao Rebuilding hippocampus neural circuit with hADSC-derived neuron cells for treating ischemic stroke Cell & bioscience, 2022-04-04;12(1):40. 2022-04-04 [PMID: 35379347] (IHC, Mouse)
• W Cheng, HL Li, SY Xi, XF Zhang, Y Zhu, Le Xing, YX Mo, MM Li, FE Kong, WJ Zhu, XG Chen, HQ Cui, ZM Cao, YF Gong, YQ Tang, Y Zhang, XY Guan, NF Ma, M Liu Growth differentiation factor 1-induced tumour plasticity provides a therapeutic window for immunotherapy in hepatocellular carcinoma Nature Communications, 2021-12-08;12(1):7142. 2021-12-08 [PMID: 34880251] (ICC, Mouse)
• Gerlach BD, Ampomah PB, Yurdagul A Et al. Efferocytosis induces macrophage proliferation to help resolve tissue injury Cell metabolism 11/10/2021 [PMID: 34784501]
• JW Opzoomer, JE Anstee, I Dean, EJ Hill, I Bouybayoun, J Caron, T Muliaditan, P Gordon, D Sosnowska, R Nuamah, SE Pinder, T Ng, F Dazzi, S Kordasti, DR Withers, T Lawrence, JN Arnold Macrophages orchestrate the expansion of a proangiogenic perivascular niche during cancer progression Science Advances, 2021-11-03;7(45):eabg9518. 2021-11-03 [PMID: 34730997] (IHC, Mouse)
• LA Bell, GJ Wallis, KS Wilcox Reactivity and increased proliferation of NG2 cells following central nervous system infection with Theiler's murine encephalomyelitis virus Journal of Neuroinflammation, 2020-12-03;17(1):369. 2020-12-03 [PMID: 33272299] (IHC, Mouse)
• Yung-Yu Yang, Chao-Ju Lin, Cheng-Chin Wang, Chieh-Min Chen, Wen-Jen Kao, Yi-Hui Chen Consecutive Hypoxia Decreases Expression of NOTCH3, HEY1, CC10, and FOXJ1 via NKX2-1 Downregulation and Intermittent Hypoxia-Reoxygenation Increases Expression of BMP4, NOTCH1, MKI67, OCT4, and MUC5AC via HIF1A Upregulation in Human Bronchial Epithelial Cells Frontiers in Cell and Developmental Biology 9/4/2020 [PMID: 33015064]
• Y Ge, Y Miao, S Gur-Cohen, N Gomez, H Yang, M Nikolova, L Polak, Y Hu, A Verma, O Elemento, JG Krueger, E Fuchs The aging skin microenvironment dictates stem cell behavior Proc. Natl. Acad. Sci. U.S.A., 2020-02-24;0(0):. 2020-02-24 [PMID: 32094197] (IHC, Mouse)
• Mohamed Ishan, Guiqian Chen, Chenming Sun, Shi‐You Chen, Yoshihiro Komatsu, Yuji Mishina et al. Increased activity of mesenchymal ALK2‐BMP signaling causes posteriorly truncated microglossia and disorganization of lingual tissues genesis 1/1/2020 [PMID: 31571391]
• Ke Liao, Fang Niu, Raghubendra Singh Dagur, Mengfan He, Changhai Tian, Guoku Hu Intranasal Delivery of lincRNA-Cox2 siRNA Loaded Extracellular Vesicles Decreases Lipopolysaccharide-Induced Microglial Proliferation in Mice Journal of Neuroimmune Pharmacology 9/1/2020 [PMID: 31325121]
• Chao Liu, Pamela Cabahug‐Zuckerman, Christopher Stubbs, Martin Pendola, Cinyee Cai, Kenneth A. Mann et al. Mechanical Loading Promotes the Expansion of Primitive Osteoprogenitors and Organizes Matrix and Vascular Morphology in Long Bone Defects Journal of Bone and Mineral Research 5/1/2019 [PMID: 30645780]
• J Kim, H Okamoto, Z Huang, G Anguiano, S Chen, Q Liu, K Cavino, Y Xin, E Na, R Hamid, J Lee, B Zambrowicz, R Unger, AJ Murphy, Y Xu, GD Yancopoulo, WH Li, J Gromada Amino Acid Transporter Slc38a5 Controls Glucagon Receptor Inhibition-Induced Pancreatic ? Cell Hyperplasia in Mice Cell Metab., 2017-06-06;25(6):1348-1361.e8. 2017-06-06 [PMID: 28591637] (ICC, Mouse)
• JC Klein, K Moses, G Zelinskyy, S Sody, J Buer, S Lang, I Helfrich, U Dittmer, CJ Kirschning, S Brandau Combined toll-like receptor 3/7/9 deficiency on host cells results in T-cell-dependent control of tumour growth Nat Commun, 2017-03-16;8(0):14600. 2017-03-16 [PMID: 28300057] (IHC, Mouse)
• Singh N, Gurav A, Sivaprakasam S, Brady E, Padia R, Shi H, Thangaraju M, Prasad P, Manicassamy S, Munn D, Lee J, Offermanns S, Ganapathy V Activation of Gpr109a, receptor for niacin and the commensal metabolite butyrate, suppresses colonic inflammation and carcinogenesis. Immunity, 2014-01-09;40(1):128-39. 2014-01-09 [PMID: 24412617] (IHC-P, Mouse)

Review for Ki67/MKI67 Antibody (AF7649) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Guo Mengqi IF/ICC Mouse 01/23/2018

Summary

• Application: Other
• Sample Tested: Mouse aortic root
• Species: Mouse
• Lot: CGVS0116042

FAQs for Ki67/MKI67 Antibody (AF7649). (Showing 1 - of FAQs).

Bioinformatics

• Gene Symbol: MKI67
• Uniprot:
  • NP_001074586()