Ki-67/MKI67 Antibody (8D5)

Images

 
Western Blot: Ki67 Antibody (8D5) [NBP2-22112] - Western blot analysis using Ki67 mouse mAb against Hela (1), MCF-7 (2) and Raji (3) cell lysate.
Immunocytochemistry/ Immunofluorescence: Ki67 Antibody (8D5) [NBP2-22112] - Ki67 antibody was tested at 1:100 in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry-Paraffin: Ki67 Antibody (8D5) [NBP2-22112] - Immunohistochemical analysis of paraffin-embedded lung cancer (left) and rectal cancer (right) using Ki67 mouse mAb with DAB staining.

Product Details

Summary
Reactivity Hu, RbSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC-P
Clone
8D5
Clonality
Monoclonal
Host
Mouse
Concentration
1.0 mg/ml

Ki-67/MKI67 Antibody (8D5) Summary

Immunogen
Synthetic peptide corresponding to an internal sequence of human Ki67 (CEDLAGFKELFQTPG), conjugated to KLH. [UniProt# P46013]
Localization
Nuclear
Marker
Proliferation Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MKI67
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
This Ki67 (8D5) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections and ELISA. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
358 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Alternate Names for Ki-67/MKI67 Antibody (8D5)

  • antigen identified by monoclonal Ki-67
  • antigen KI-67
  • Ki67
  • Ki-67
  • Ki-67/MKI67
  • KIA
  • Marker Of Proliferation Ki-67
  • MIB-1
  • MKI67
  • PPP1R105
  • proliferation-related Ki-67 antigen
  • Protein Phosphatase 1
  • Regulatory Subunit 105
  • TSG126

Background

Ki67, also known as MKI67, it is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol MKI67
Entrez
Uniprot

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