Recombinant Human KGF/FGF-7 Animal-Free Protein

SDS-Page: Recombinant Human KGF/FGF-7 Animal-Free Protein [NBP2-76197] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel.

Bioactivity: Recombinant Human KGF/FGF-7 Animal-Free Protein [NBP2-76197] - KGF/FGF-7 induced proliferation of 4MBR5 cells.

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Animal Free
• Concentration: LYOPH

Recombinant Human KGF/FGF-7 Animal-Free Protein Summary

• Description: An un-tagged bioactive recombinant protein corresponding to Human KGF/FGF-7
  
  

  Source: E. coli

  Amino Acid Sequence: MACNDMTPEQM ATNVNCSSPE RHTRSYDYME GGDIRVRRLF CRTQWYLRID KRGKVKGTQE MKNNYNIMEI RTVAVGIVAI KGVESEFYLA MNKEGKLYAK KECNEDCNFK ELILENHYNT YASAKWTHNG GEMFVALNQK GIPVRGKKTK KEQKTAHFLP MAIT

  Produced with no materials of animal or human origin using ANIMAL-FREE purification processes.

  Production and Purification

  • No animal-derived ingredients, No FBS
  • E. coli based production system
  • Animal-Free facility, fermentor, columns, filters, labware, lyophilization, and storage
  
  

  Quality Assurance

  • Endotoxin levels at <1 EUs/ug
  • Purity levels at 95% or greater
• Specificity: KGF/FGF-7 is a monomer, 19 kDa (164 aa)
• Preparation Method: Protein is derived from genetically modified E. coli.
• Details of Functionality: 4MBr-5 cell proliferation, >1.7 x 10^4 units/mg (ED50 acceptance criteria are <60 ng/mL).
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: FGF7
• Purity: >90%, by SDS-PAGE
• Endotoxin Note: Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 20 mM NaP, 0.5 M NaCl, pH 7.5
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >90%, by SDS-PAGE
• Reconstitution Instructions: Sterile water at 0.1 mg/mL. If a precipitate is observed, centrifuge the solution thoroughly and use only the soluble fraction (removing it from the precipitate). A 10% overfill has been added to compensate for any loss of protein in the precipitate.

Notes

Lot Specific Information:
Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.

Detailed Reconstitution:
Centrifuge vial before opening. Suspend the product by gently pipetting the recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots.

Expiration Date:
12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.

Alternate Names for Recombinant Human KGF/FGF-7 Animal-Free Protein

• FGF7
• FGF-7
• fibroblast growth factor 7HBGF-7
• HBGF7
• HBGF-7
• Heparin-binding growth factor 7
• keratinocyte growth factor
• KGF
• KGFfibroblast growth factor 7 (keratinocyte growth factor)

Background

Keratinocyte Growth Factor (KGF, FGF-7, HBGF-7) is a member of the FGF family of 23 related mitogenic proteins. These proteins play a central role during embryonic development and postnatal growth and tissue repair, by promoting cellular proliferation and differentiation. KGF specifically stimulates epithelial cells and keratinocytes, it is the most potent growth factor identified thus far for skin keratinocytes. KGF takes part in the development of kidney and lung, angiogenesis and has a key role in wound healing following skin injuries. It shows considerable species cross-reactivity (mouse, monkey, porcine cells) as the other members of the FGF family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FGF7