Human KGF/FGF-7 ELISA Kit (Colorimetric) Summary
| Sensitivity |
0.5 pg/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 12% |
| Intra-Assay |
CV% < 10% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
FGF7 |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
FGF7 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FGF7. |
Reactivity Notes
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Assay Diluent (Item E2): 5x concentrated buffer.
- Detection Antibody FGF-7 (Item F): Biotinylated anti-human FGF-7. Each vialis enough to assay half the microplate.
- FGF-7 Microplate (Item A): 96 wells coated with anti-human FGF-7
- HRP-Streptavidin concentrates (Item G): 200x concentrated HRP-conjugatedstreptavidin.
- Standard Protein (Item C): Human FGF-7. 1 vial is enough to run eachstandard in duplicate.
- Stop Solution (Item I): 0.2 M sulfuric acid.
- TMB One-Step Substrate Reagent (Item H): 3, 3’, 5, 5’- tetramethylbenzidine(TMB) in buffered solution.
- Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human KGF/FGF-7 ELISA Kit (Colorimetric)
Background
Keratinocyte Growth Factor (KGF, FGF-7, HBGF-7) is a member of the FGF family of 23 related mitogenic proteins. These proteins play a central role during embryonic development and postnatal growth and tissue repair, by promoting cellular proliferation and differentiation. KGF specifically stimulates epithelial cells and keratinocytes, it is the most potent growth factor identified thus far for skin keratinocytes. KGF takes part in the development of kidney and lung, angiogenesis and has a key role in wound healing following skin injuries. It shows considerable species cross-reactivity (mouse, monkey, porcine cells) as the other members of the FGF family.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for KGF/FGF-7 ELISA Kit (KA1714). (Showing 1 - 1 of 1 FAQ).
-
wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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