KGF/FGF-7 Antibody (29522) [Unconjugated]

Recombinant Human KGF/FGF‑7 (Catalog # 251-KG) stimulates proliferation in the 4MBr‑5 rhesus monkey epithelial cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human KGF/FGF‑7 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human KGF/FGF‑7 Monoclonal Antibody (Catalog # MAB251). The ND50 is typically 0.1-0.5 µg/mL.

• Reactivity: Hu
• Applications: B/N, ELISA(Cap)
• Clone: 29522
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

KGF/FGF-7 Antibody (29522) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human KGF/FGF‑7
  Cys32-Thr194
  Accession # P21781
• Specificity: Detects human KGF/FGF‑7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) FGF-3, rhFGF-4, rhFGF-5, rhFGF-6, rhFGF acidic, or rhFGF basic is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FGF7
• Purity Statement: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.1-0.5 ug/mL
• Application Notes: ELISA Detection: Human KGF/FGF-7 Biotinylated Antibody (Catalog number BAF251)
  Standard: Recombinant Human KGF/FGF-7 (Catalog number 251-KG)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KGF/FGF-7 Antibody (29522) [Unconjugated]

• FGF7
• FGF-7
• fibroblast growth factor 7HBGF-7
• HBGF7
• HBGF-7
• Heparin-binding growth factor 7
• keratinocyte growth factor
• KGF
• KGFfibroblast growth factor 7 (keratinocyte growth factor)

Background

KGF (keratinocyte growth factor), also known as FGF-7 (fibroblast growth factor-7), is one of 22 known members of the mouse FGF family of secreted proteins that plays a key role in development, morphogenesis, angiogenesis, wound healing, and tumorigenesis (1‑4). KGF expression is restricted to cells of mesenchymal origin. When secreted, it acts as a paracrine growth factor for nearby epithelial cells (1). KGF speeds wound healing by being dramatically upregulated in response to damage to skin or internal structures that results in high local concentrations of inflammatory mediators such as IL-1 and TNF-alpha . (2, 5). KGF promotes cell migration and invasion, and mediates melanocyte transfer to keratinocytes upon UVB radiation (6, 7). It has been used ectopically to avoid chemotherapy-induced oral mucositis in patients with hematological malignancies (1). Deletion of KGF affects kidney development, producing abnormally small ureteric buds and fewer nephrons (8). It also impedes hair follicle differentiation (9). The 194 amino acid (aa) KGF precursor contains a 31 aa signal sequence and, like all other FGFs, an ~120 aa beta -trefoil scaffold that includes receptor- and heparin-binding sites. KGF signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (FGF R2-IIIb/KGF R) (10). Receptor dimerization requires an octameric or larger heparin or heparin sulfate proteoglycan (11). FGF-10, also called KGF2, shares 51% aa identity and similar function to KGF, but shows more limited expression than KGF and uses an additional receptor, FGF R2-IIIc (12). Following receptor engagement, KGF is typically degraded, while FGF-10 is recycled (12). Mature human KGF, which is active across species, shares 98% aa sequence identity with bovine, equine, ovine, and canine, 96% with mouse and porcine, and 92% with rat KGF, respectively.

1. Finch, P.W. and J.S. Rubin (2006) J. Natl. Cancer Inst. 98:812.
2. Werner, S. et al. (2007) J. Invest. Dermatol. 127:998.
3. Werner, S. (1998) Cytokine Growth Factor Rev. 9:153.
4. Mason, I.J. et al. (1994) Mech. Dev. 45:15.
5. Geer, D.J. et al. (2005) Am. J. Pathol. 167:1575.
6. Niu, J. et al. (2007) J. Biol. Chem. 282:6001.
7. Cardinali, G. et al. (2005) J. Invest. Dermatol. 125:1190.
8. Qiao, J. et al. (1999) Development 126:547.
9. Guo, L. et al. (1996) Genes Dev. 10:165.
10. de Georgi, V. et al. (2007) Dermatol. Clin. 25:477.
11. Hsu, Y-R. et al. (1999) Biochemistry 38:2523.
12. Belleudi, F. et al. (2007) Traffic 8:1854.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KGF/FGF-7 Antibody (MAB251)(3)

Publications using MAB251

• Sohinee Bhattacharyya, Chet Oon, Aayush Kothari, Wesley Horton, Jason Link, Rosalie C. Sears et al. Acidic fibroblast growth factor underlies microenvironmental regulation of MYC in pancreatic cancer Journal of Experimental Medicine 2020-08-03 [PMID: 32434218] (Neutralization, Human)
• Steinberg Z, Myers C, Heim VM, Lathrop CA, Rebustini IT, Stewart JS, Larsen M, Hoffman MP FGFR2b signaling regulates ex vivo submandibular gland epithelial cell proliferation and branching morphogenesis. Development, 2005-02-16;132(6):1223-34. 2005-02-16 [PMID: 15716343] (Neutralization, Mouse)
• Putnins EE, Sanaie AR, Wu Q Induction of keratinocyte growth factor 1 Expression by lipopolysaccharide is regulated by CD-14 and toll-like receptors 2 and 4. Infect. Immun., 2002-12-01;70(12):6541-8. 2002-12-01 [PMID: 12438323] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: FGF7
• Uniprot:
  • P21781()