Keap1 Antibody

Images

 
Western Blot: KEAP1 Antibody [NBP2-17036] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Keap1 Antibody [NBP2-17036] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KEAP1 protein stained by KEAP1 antibody [C1C3] diluted at 1:500. Blue: ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, PmSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Keap1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human KEAP1. The exact sequence is proprietary.
Localization
Cytoplasm; Nucleus
Predicted Species
Mouse (96%), Rat (96%), Porcine (99%), Primate (100%), Bovine (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KEAP1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Keap1 Antibody

  • INrf2
  • INrf2MGC9454
  • Keap1
  • kelch-like ECH-associated protein 1
  • Kelch-like protein 19
  • KIAA0132MGC4407
  • KLHL19Cytosolic inhibitor of Nrf2
  • MGC10630
  • MGC1114
  • MGC20887

Background

This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol KEAP1
Entrez
Uniprot