KDM2A/FBXL11 Antibody [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: CoraFluor 1

KDM2A/FBXL11 Antibody [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: A synthetic peptide made to an N-terminal portion of the human KDM2A/FBXL11 protein (between residues 350-500) [UniProt Q9Y2K7]
• Localization: Nucleus, nucleoplasm. Note: Punctate expression throughout the nucleoplasm and enriched in the perinucleolar region. Specifically nucleates at CpG islands where it's presence results in chromatin depleted in H3K36me2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KDM2A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for KDM2A/FBXL11 Antibody [CoraFluor™ 1]

• [Histone-H3]-lysine-36 demethylase 1A
• CXXC8
• CXXC8F-box protein FBL7
• CXXC-type zinc finger protein 8
• EC 1.14.11.27
• FBL11
• FBL11DKFZp434M1735
• FBL7
• FBL7F-box protein Lilina
• F-box and leucine-rich repeat protein 11DKFZP434M1735
• F-box protein FBL11
• F-box protein FBL7
• FBXL11
• FBXL11FLJ46431
• FLJ00115
• JHDM1A
• JHDM1AF-box/LRR-repeat protein 11
• JmjC domain-containing histone demethylation protein 1A
• jumonji C domain-containing histone demethylase 1A
• KDM2A
• KDM2A/FBXL11
• KIAA1004
• KIAA1004EC 1.14.11.27
• LILINA
• lysine (K)-specific demethylase 2A
• lysine-specific demethylase 2A
• SF-KDM2A

Background

KDM2A/FBXL11 (F-box and leucine-rich repeat protein 11) is a histone demethylase which has an important role in histone code as well as epigenetic silencing. KDM2A/FBXL11 is known to have interactions with SMAD7, SMAD3, ZNF512B, HIST2H3A and HIST2H3C. KDM2A/FBXL11 has been studied in relation to anemia and Fanconi's anemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KDM2A