KDM2A/FBXL11 Antibody [DyLight 650]

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: DyLight 650

KDM2A/FBXL11 Antibody [DyLight 650] Summary

• Immunogen: A synthetic peptide made to an N-terminal portion of the human KDM2A/FBXL11 protein (between residues 350-500) [UniProt Q9Y2K7]
• Localization: Nucleus, nucleoplasm. Note: Punctate expression throughout the nucleoplasm and enriched in the perinucleolar region. Specifically nucleates at CpG islands where it's presence results in chromatin depleted in H3K36me2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KDM2A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for KDM2A/FBXL11 Antibody [DyLight 650]

• [Histone-H3]-lysine-36 demethylase 1A
• CXXC8
• CXXC8F-box protein FBL7
• CXXC-type zinc finger protein 8
• EC 1.14.11.27
• FBL11
• FBL11DKFZp434M1735
• FBL7
• FBL7F-box protein Lilina
• F-box and leucine-rich repeat protein 11DKFZP434M1735
• F-box protein FBL11
• F-box protein FBL7
• FBXL11
• FBXL11FLJ46431
• FLJ00115
• JHDM1A
• JHDM1AF-box/LRR-repeat protein 11
• JmjC domain-containing histone demethylation protein 1A
• jumonji C domain-containing histone demethylase 1A
• KDM2A
• KDM2A/FBXL11
• KIAA1004
• KIAA1004EC 1.14.11.27
• LILINA
• lysine (K)-specific demethylase 2A
• lysine-specific demethylase 2A
• SF-KDM2A

Background

KDM2A / FBXL11 (also known as JHDM1A, lysine specific demethylase 1A) is a F-box family member and JmjC-domain containing histone demethylase that specifically demethylates Lysine 36 of H3 (H3 K36). KDM2A / FBXL11 is involved in epigenetic silencing and required to maintain the heterochromatic state, as well as sustaining centromeric integrity and genomic stability during mitosis (PMID 19001877). Under conditions of starvation, FBXL11's ability to repress RNA transcription has been shown to be reduced (PMID 20379134). In addition, KDM2A / FBXL11 is also a key inhibitory regulator of NF kappa B activity. This regulation occurs when K218 and K221 of the p65 subunit are methylated. The overexpression of KDM2A / FBXL11 can decrease the viability of specific cancer cell lines, while downregulation was shown to have the opposite effect (PMID 20080798). KDM2A / FBXL11 shows further regulatory involvement at CpG islands. Nonmethylated CpG islands preferentially bind KDM2A / FBXL11 using a zinc finger domain, in which the binding is blocked when CpG DNA is in its methlylated form (PMID 20417597).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KDM2A