Home » CXXC4 » CXXC4 Antibodies » CXXC4 Antibody - BSA Free

CXXC4 Antibody - BSA Free

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Western Blot: CXXC4 Antibody [NBP1-76491] - Human brain tissue lysate with CXXC4 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Western Blot: CXXC4 Antibody [NBP1-76491] - Human brain tissue lysate with CXXC4 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry-Paraffin: CXXC4 Antibody [NBP1-76491] - Human brain tissue with CXXC4 antibody at 10 ug/mL.
Immunofluorescence: CXXC4 Antibody [NBP1-76491] - Immunofluorescence of CXXC4 in human brain tissue with CXXC4 antibody at 20 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-76491-0.025mg
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CXXC4 Antibody - BSA Free Summary

Immunogen
Antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human CXXC4. The immunogen is located within amino acids 50 - 100 of CXXC4. Amino Acid Squence: NLPERVGTFSAIPALGG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CXXC4
Purity
Peptide affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunofluorescence 20 ug/ml
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1 ug/ml
Control Peptide
CXXC4 Peptide (NBP1-76491PEP)
Publications
Read Publication using
NBP1-76491 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for CXXC4 Antibody - BSA Free

  • CXXC finger protein 4
  • CXXC-type zinc finger protein 4
  • Dvl-binding protein IDAX (inhibition of the Dvl and Axin complex)
  • IDAXCXXC finger 4
  • Inhibition of the Dvl and axin complex protein
  • MGC149872

Background

CXXC4 is zinc finger protein that binds directly to the PDZ domain of DVL1, a protein involved in the Wnt signaling pathway, through its C-terminal region. CXXC4's interaction with DVL1 competes with DVL1-AXIN binding, although the affinity of DVL1 for CXXC4 is lower than that for AXIN. In mouse fibroblasts, CXXC4 suppressed Wnt3a induction of beta-catenin and inhibited Wnt3a-dependent TCF4 activation, suggesting that CXXC4 acts as a negative regulator of the Wnt signaling pathway and functions between DVL and beta-catenin. Recent reports suggest that decreased CXXC4 expression is associated with kidney cancer metastasis, cell proliferation, reduced apoptosis in response to cancer drugs, and upregulation of genes involved with proliferation, invasion and cell survival, suggesting that CXXC4 plays a critical role in tumor progression in kidney cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Flow Cytometry: beta-Catenin Antibody (12F7) [NBP1-54467] - An intracellular stain was performed on SK-MEL-28 cells with beta-Catenin Antibody [12F7] NBP1-54467B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).
Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - Analysis of beta Catenin expression in 1) HepG2, 2) MCF7, and 3) Cos7 whole cell lysates using NBP1-54467.
Immunohistochemistry: beta-Catenin Antibody (12F7) [NBP1-54467] - IF beta-Catenin staining of mouse brain tissue. This image submitted by a verified customer review.
(4 Reviews)
(14 Publications)
Species: Ch, Av-Du, Hu, Mu, Pm, Rt
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
INTS6 Antibody
NBP1-85302
Immunocytochemistry/Immunofluorescence: INTS6 Antibody [NBP1-85302] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: INTS6 Antibody [NBP1-85302] - Analysis in human testis and skeletal muscle tissues. Corresponding INTS6 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: INTS6 Antibody [NBP1-85302] - Staining of human placenta shows moderate nuclear positivity in trophoblastic cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
CXXC5 Antibody -
BSA Free
NBP1-76513
Immunocytochemistry/Immunofluorescence: CXXC5 Antibody [NBP1-76513] - Immunofluorescence of CXXC5 in Human Brain cells with CXXC5 antibody at 20 ug/mL.
Western Blot: CXXC5 Antibody [NBP1-76513] - Human brain tissue lysate with CXXC5 antibody at 1 ug/mL.
Immunohistochemistry: CXXC5 Antibody [NBP1-76513] - Immunohistochemistry of CXXC5 in human brain tissue with CXXC5 antibody at 2.5 ug/mL.
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
Axin-1 Antibody
AF3287
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Axin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3287) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Axin-1 at approximately 120 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
(1 Review)
(8 Publications)
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Dishevelled-1
Antibody
NBP2-24648
Western Blot: Dishevelled-1 Antibody [NBP2-24648] - Analysis of Dishevelled-1 in Raji cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Species: Hu, Mu, Pm, Rt
Applications: WB
DNMT1 Antibody
(60B1220.1) - BSA Fre
NB100-56519
Flow Cytometry: DNMT1 Antibody (60B1220.1) [NB100-56519] - Analysis of PE conjugate of NB100-56519. An intracellular stain was performed on HeLa cells with DNMT1 antibody (60B1220.1) NB100-56519PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0
Flow Cytometry: DNMT1 Antibody (60B1220.1) [NB100-56519] - An intracellular stain was performed on HeLa cells with DNMT1 [60B1220.1] Antibody NB100-56519AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Immunoprecipitation: DNMT1 Antibody (60B1220.1) [NB100-56519] - The PCNAS228I mutation differentially affects PCNA binding to PIP box-containing proteins. GST-PIP pull down of His-S-PCNAWT or PCNAS228I. Figure shows Coomassie stained gel of representative pull down (top) and anti-PCNA western blot of the same samples diluted 1:20 (bottom). Amount of 'input' loaded for Coomassie is equivalent to 1%, 'Glut. beads' (Glutathione sepharose 4B beads) is equivalent to 25%. Molecular weight markers are indicated. Image collected and cropped by CiteAb from the following publication (http://linkinghub.elsevier.com/retrieve/pii/S1568786416304013), licensed under a CC-BY licence.
(4 Reviews)
(124 Publications)
Species: Bv, Hu, Mu, Po, Rt, Sh
Applications: ChIP, CyTOF-ready, Flow-IC, Flow, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, KD, Simple Western, WB
MBD1 Antibody
(100B272.1)
NB100-56537
Knockdown Validated: MBD1 Antibody (100B272.1) [NB100-56537] - blot shows lysates of HEK293T human embryonic kidney parental cell line and MBD1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2.0 ug/ml of Mouse Anti-Human MBD1 Monoclonal Antibody (Catalog # NB100-56537) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for MBD1 at approximately 60 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.
Immunocytochemistry/Immunofluorescence: MBD1 Antibody (100B272.1) [NB100-56537] - HeLa cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-MBD1 (100B272.1) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunocytochemistry/Immunofluorescence: MBD1 Antibody (100B272.1) [NB100-56537] - NIH3T3 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-MBD1 (100B272.1) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
(2 Reviews)
(8 Publications)
Species: Hu, Mu
Applications: ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, WB
TCF7L1/TCF3
Antibody
AF6116
TCF7L1 was detected in immersion fixed BG01V human embryonic stem cells using Human TCF7L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6116) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green, upper panel; Catalog # NL012) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Western blot shows lysates of human pancreas and human lung tissue. PVDF Membrane was probed with 0.2 µg/mL of Human TCF7L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6116) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TCF7L1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu
Applications: ICC, WB
Cyclin D1 Antibody
(SPM587)
NBP2-32840
Western Blot: Cyclin D1 Antibody (SPM587) [NBP2-32840] - HELLS expression and protein levels are modulated with YAP1/TEAD inhibition downstream of SHH signaling. Western blot showing protein levels of HELLS, cleaved caspase 3 (CC3), and Cyclin D1 in verteporfin treated CGNPs. Blot is representative of three replicates. Full-length blots are presented in Supplementary Fig. S4. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-019-50088-1), licensed under a CC-BY licence.
Flow Cytometry: Cyclin D1 Antibody (SPM587) [NBP2-32840] - Flow Cytometric analysis of human Cyclin D1 on Jurkat cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Cyclin D1 Monoclonal Antibody (SPM587).
Flow Cytometry: Cyclin D1 Antibody (SPM587) [NBP2-32840] - Flow Cytometric analysis of human Cyclin D1 on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Cyclin D1 Monoclonal Antibody (SPM587).
(1 Review)
(7 Publications)
Species: Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Dact1 Antibody
NBP2-24768
Western Blot: Dact1 Antibody [NBP2-24768] - Analysis of Dapper homolog 1 in brain lysate. Lane 1 shows pre-immune sera. Lanes 2, 3 and 4 show this antibody at 5 ug/mL and rat brain (0.5 ug/mL) lysate.
Immunohistochemistry-Paraffin: Dact1 Antibody [NBP2-24768] - FFPE human brain stained with Dapper homolog 1 antibody at 10 ug/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
(1 Publication)
Species: Ch, Hu, Mu, Pm, Rt, Xp, Ze
Applications: IHC, IHC-P, WB
KDM2B Antibody
(5G1)
H00084678-M09
Western Blot: KDM2B Antibody (5G1) [H00084678-M09] - Western Blot analysis of FBXL10 expression in Hela NE ( Cat # L013V3 ).
Sandwich ELISA: KDM2B Antibody (5G1) [H00084678-M09] - Detection limit for recombinant GST tagged FBXL10 is approximately 0.1ng/ml as a capture antibody.
Immunocytochemistry/Immunofluorescence: KDM2B Antibody (5G1) [H00084678-M09] - Analysis of monoclonal antibody to FBXL10 on HeLa cell. Antibody concentration 10 ug/ml
(3 Publications)
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA, WB
KDM2A/FBXL11
Antibody - BSA Free
NBP1-78305
Immunocytochemistry/Immunofluorescence: KDM2A/FBXL11 Antibody [NBP1-78305] - Human astrocytoma cell line lentivirally transduced and expressing KDM2A. Cells were fixed with 4% paraformaldehyde and stained with anti-KDM2A, followed by Alexa Fluor 488-anti-rabbit secondary antibody. DAPI staining shows nuclei. ICC/IF image submitted by a verified customer review.
Western Blot: KDM2A/FBXL11 Antibody [NBP1-78305] - 1) 293T. 2) 293T transiently transfected to express human KDM2A. WB image submitted by a verified customer review.
Immunohistochemistry: KDM2A/FBXL11 Antibody [NBP1-78305] - IHC analysis of KDM2A / FBXL11 in human kidney cancer xenograft using DAB with hematoxylin counterstain.
(3 Reviews)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
TET1 Antibody -
BSA Free
NBP3-11863
Western Blot: TET1 Antibody [NBP3-11863] - Total protein from human K562 Nuclear and mouse L929 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TET1 (NBP3-11863) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).
Immunohistochemistry-Paraffin: TET1 Antibody [NBP3-11863] - Analysis of a FFPE tissue section of human placenta using 1:200 dilution of TET1 antibody (NBP3-11863). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
Flow (Intracellular): TET1 Antibody [NBP3-11863] - An intracellular stain was performed on A431 cells with NBP3-11863 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).
Species: Hu, Mu
Applications: Flow-IC, ICC/IF, IHC, IHC-P, WB
beta-Catenin
Antibody (12F7) - BSA
NBP1-54467
Flow Cytometry: beta-Catenin Antibody (12F7) [NBP1-54467] - An intracellular stain was performed on SK-MEL-28 cells with beta-Catenin Antibody [12F7] NBP1-54467B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).
Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - Analysis of beta Catenin expression in 1) HepG2, 2) MCF7, and 3) Cos7 whole cell lysates using NBP1-54467.
Immunohistochemistry: beta-Catenin Antibody (12F7) [NBP1-54467] - IF beta-Catenin staining of mouse brain tissue. This image submitted by a verified customer review.
(4 Reviews)
(14 Publications)
Species: Ch, Av-Du, Hu, Mu, Pm, Rt
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
NKD1 Antibody
NBP1-55270
Western Blot: NKD1 Antibody [NBP1-55270] - HepG2 tissue lysate at a concentration of 1ug/ml.
Immunohistochemistry-Paraffin: NKD1 Antibody [NBP1-55270] - Human Lung Alveolar cells (indicated with arrows), 4-8ug/ml.
Species: Hu
Applications: IHC, IHC-P, WB
ASXL1 Antibody
(6E2)
H00171023-M05
Western Blot: ASXL1 Antibody (6E2) [H00171023-M05] - Analysis of ASXL1 expression in MCF-7 (Cat # L046V1).
Western Blot: ASXL1 Antibody (6E2) [H00171023-M05] - Functional effects of CRISPR/Cas9-mediated ASXL1 mutation correction. Evaluation of ASXL1 protein expression by Western blotting. Left-hand side: ASXL1 protein expression in the SET2 leukemia cell line (wild-type for ASXL1), uncorrected KBM5 cells, the K562 leukemia cell line (carrying the Y591X heterozygous ASXL1 mutation), and KBM5 clones (labeled 1-5) with heterozygous precise correction of the ASXL1 mutation. Right-hand side: ASXL1 protein expression in the SET2 leukemia cell line (wild-type for ASXL1), uncorrected KBM5 cells, and KBM5 clones (labeled 1-5) with homozygous precise correction of the ASXL1 mutation. Beta-actin was used as loading control. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/6392) licensed under a CC-BY licence.
ELISA: ASXL1 Antibody (6E2) [H00171023-M05] - Detection limit for recombinant GST tagged ASXL1 is 0.3 ng/ml as a capture antibody.
(3 Publications)
Species: Hu
Applications: ELISA, IP, WB
ASXL2 Antibody
H00055252-B01P
Immunocytochemistry/Immunofluorescence: ASXL2 Antibody [H00055252-B01P] - Analysis of purified antibody to ASXL2 on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: ASXL2 Antibody [H00055252-B01P] - Analysis of ASXL2 expression in transfected 293T cell line by ASXL2 polyclonal antibody. Lane 1: ASXL2 transfected lysate(100.98 KDa). Lane 2: Non-transfected lysate.
Species: Hu
Applications: WB
CXXC1 Antibody
NBP2-92895
Immunocytochemistry/Immunofluorescence: CXXC1 Antibody [NBP2-92895] - Analysis of A549 cells using CXXC1 . Blue: DAPI for nuclear staining.
Western Blot: CXXC1 Antibody [NBP2-92895] - Analysis of extracts of various cell lines, using CXXC1 .Exposure time: 90s.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB

Publications for CXXC4 Antibody (NBP1-76491)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Publication using NBP1-76491 Applications Species
Olofsen P, Fatrai S, van Strien P et al. Malignant Transformation Involving CXXC4 Mutations Identified in a Leukemic Progression Model of Severe Congenital Neutropenia Cell Reports Medicine Jan 1 2020 (WB, Human) WB Human

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Bioinformatics

Gene Symbol CXXC4
Uniprot