KDM2B Antibody (5G1)

Images

 
Western Blot: KDM2B Antibody (5G1) [H00084678-M09] - Western Blot analysis of FBXL10 expression in Hela NE ( Cat # L013V3 ).
Immunocytochemistry/ Immunofluorescence: KDM2B Antibody (5G1) [H00084678-M09] - Analysis of monoclonal antibody to FBXL10 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: KDM2B Antibody (5G1) [H00084678-M09] - Detection limit for recombinant GST tagged FBXL10 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, S-ELISA
Clone
5G1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

KDM2B Antibody (5G1) Summary

Immunogen
FBXL10 (NP_115979, 457 a.a. ~ 555 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LGKKPKAPALRFLKRTLSNESEESVKSTTLAVDYPKTPTGSPATEVSAKWTHLTEFELKGLKALVEKLESLPENKKCVPEGIEDPQALLEGVKNVLKEH
Specificity
FBXL10 - F-box and leucine-rich repeat protein 10
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KDM2B
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Publications
Read Publications using
H00084678-M09 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for KDM2B Antibody (5G1)

  • [Histone-H3]-lysine-36 demethylase 1B
  • CXXC2
  • CXXC2F-box protein FBL10
  • CXXC-type zinc finger protein 2
  • FBL10
  • F-box and leucine-rich repeat protein 10JEMMA (Jumonji domain, EMSY-interactor, methyltransferase motif) protein
  • F-box protein FBL10
  • FBXL10
  • JHDM1B
  • JHDM1BF-box/LRR-repeat protein 10
  • JmjC domain-containing histone demethylation protein 1B
  • jumonji C domain-containing histone demethylase 1B
  • Jumonji domain-containing EMSY-interactor methyltransferase motif protein
  • KDM2B
  • lysine (K)-specific demethylase 2B
  • lysine-specific demethylase 2B
  • NDY1
  • PCCX2
  • PCCX2EC 1.14.11.27
  • protein containing CXXC domain 2
  • Protein JEMMA
  • Protein-containing CXXC domain 2

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for KDM2B Antibody (H00084678-M09)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol KDM2B
Entrez
OMIM
Uniprot