Biological Strategies: Western Blot: KDM2A/FBXL11 Antibody [NB100-74602] - Detection of Human JHDM1A by Western Blot. Samples: Whole cell lysate from HeLa (50, 15, 5 ug) and 293T (50 ug) cells prepared using NETN ...read more
Immunohistochemistry: KDM2A/FBXL11 Antibody [NB100-74602] - Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti- JHDM1A used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Independent Antibodies: Western Blot: KDM2A/FBXL11 Antibody [NB100-74602] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-74602 used for WB at 0.04 mcg/ml (A) ...read more
Immunohistochemistry-Paraffin: KDM2A/FBXL11 Antibody [NB100-74602] - analysis of human lung tumor tissue using anti-KDM2A antibody. Image from verified customer review.
The immunogen recognized by this antibody maps to a region between residue 825 and 875 of human JmjC domain-containing histone demethylation protein 1A using the numbering given in entry NP_036440.1 (GeneID 22992).
Immunogen affinity purified
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Immunohistochemistry 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
133 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 2 Reviews rated 5 using NB100-74602 in the following applications:
F-box and leucine-rich repeat protein 11DKFZP434M1735
F-box protein FBL11
JHDM1AF-box/LRR-repeat protein 11
JmjC domain-containing histone demethylation protein 1A
jumonji C domain-containing histone demethylase 1A
lysine (K)-specific demethylase 2A
lysine-specific demethylase 2A
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains at least 6 highly degenerated leucine-rich repeats.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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