KDM2A/FBXL11 Antibody

Images

 
Western Blot: KDM2A/FBXL11 Antibody [NB100-74602] - Detection of Human JHDM1A by Western Blot. Samples: Whole cell lysate from HeLa (50, 15, 5 ug) and 293T (50 ug) cells prepared using NETN lysis buffer. Antibodies: ...read more
Immunohistochemistry: KDM2A/FBXL11 Antibody [NB100-74602] - Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti- JHDM1A used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: KDM2A/FBXL11 Antibody [NB100-74602] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-74602 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for ...read more
Immunohistochemistry-Paraffin: KDM2A/FBXL11 Antibody [NB100-74602] - analysis of human lung tumor tissue using anti-KDM2A antibody. Image from verified customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

KDM2A/FBXL11 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 825 and 875 of human JmjC domain-containing histone demethylation protein 1A using the numbering given in entry NP_036440.1 (GeneID 22992).
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDM2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
Theoretical MW
133 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-74602 in the following applications:

Publications
Read Publications using
NB100-74602 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for KDM2A/FBXL11 Antibody

  • [Histone-H3]-lysine-36 demethylase 1A
  • CXXC8F-box protein FBL7
  • CXXC-type zinc finger protein 8
  • FBL11DKFZp434M1735
  • FBL7F-box protein Lilina
  • F-box and leucine-rich repeat protein 11DKFZP434M1735
  • F-box protein FBL11
  • FBXL11FLJ46431
  • FLJ00115
  • JHDM1AF-box/LRR-repeat protein 11
  • JmjC domain-containing histone demethylation protein 1A
  • jumonji C domain-containing histone demethylase 1A
  • KIAA1004EC 1.14.11.27
  • LILINA
  • lysine (K)-specific demethylase 2A
  • lysine-specific demethylase 2A
  • SF-KDM2A

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains at least 6 highly degenerated leucine-rich repeats.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for KDM2A/FBXL11 Antibody (NB100-74602)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Reviews for KDM2A/FBXL11 Antibody (NB100-74602) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 2 application: ICC, WB.

Reviews using NB100-74602:
Filter by Applications
ICC
(1)
WB
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry KDM2A/FBXL11 NB100-74602
Enlarge
reviewed by:
Hunain Alam
ICC Human 10/08/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman lung tumor
SpeciesHuman
LotA1

Blocking

Blocking Details10% Normal Goat Serum

Primary Anitbody

Dilution Ratio1:50

Secondary Antibody

Secondary DescriptionAnti-rabbit-Biotin
Secondary Manufacturer Cat#Vector Lab
Secondary Concentration1:200

Details

Detection NotesAEC reagent from Vector
Fixation DetailsParafin-fixed tissue
Wash DescriptionPBS
Western Blot KDM2A/FBXL11 NB100-74602
Enlarge
reviewed by:
Hunain Alam
WB Human 10/08/2015
View

Summary

ApplicationWestern Blot
Sample TestedLung cell lines whole cell lysate
SpeciesHuman
LotA1

Blocking

Blocking Details5% Milk

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary Descriptionanti-rabbit IgG-HRP
Secondary Manufacturer Cat#SantaCruz
Secondary Concentration1:5000

Details

Detection NotesECL

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for KDM2A/FBXL11 Antibody (NB100-74602) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for KDM2A/FBXL11 Antibody (NB100-74602)

Discover related pathways, diseases and genes to KDM2A/FBXL11 Antibody (NB100-74602). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

5
5
2
4
0
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0
2
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1
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Hunain Alam
10/08/2015
Application: ICC
Species: Human

Hunain Alam
10/08/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol KDM2A
Entrez
Uniprot