KCC2/SLC12A5 Antibody (HL2535) - Azide and BSA Free

Western Blot: KCC2/SLC12A5 Antibody (HL2535) - Azide and BSA Free [NBP3-25541] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody [HL2535] (NBP3-25541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western Blot: KCC2/SLC12A5 Antibody (HL2535) - Azide and BSA Free [NBP3-25541] - Unboiled and boiled various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KCC2 antibody [HL2535] (NBP3-25541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: KCC2/SLC12A5 Antibody (HL2535) - Azide and BSA Free [NBP3-25541] - KCC2 antibody [HL2535] detects KCC2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. KCC2 stained by KCC2 antibody [HL2535] (NBP3-25541) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: KCC2/SLC12A5 Antibody (HL2535) - Azide and BSA Free [NBP3-25541] - KCC2 antibody [HL2535] detects KCC2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. KCC2 stained by KCC2 antibody [HL2535] (NBP3-25541) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody [HL2535] (NBP3-25541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

KCC2/SLC12A5 antibody [HL2535] detects KCC2/SLC12A5 protein at Glogi by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: KCC2/SLC12A5 stained by KCC2/SLC12A5 antibody [HL2535] (NBP3-25541) diluted at 1:250.Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI .

• Reactivity: Mu
• Applications: WB, IHC
• Clone: HL2535
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

KCC2/SLC12A5 Antibody (HL2535) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit KCC2/SLC12A5 Antibody (HL2535) - Azide and BSA Free (NBP3-25541) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide of mouse KCC2/SLC12A5
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SLC12A5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for KCC2/SLC12A5 Antibody (HL2535) - Azide and BSA Free

• Electroneutral potassium-chloride cotransporter 2
• hKCC2
• KCC2
• KCC2K-Cl cotransporter 2
• KIAA1176erythroid K-Cl cotransporter 2
• Neuronal K-Cl cotransporter
• SLC12A5
• solute carrier family 12 (potassium/chloride transporter), member 5
• solute carrier family 12 member 5

Background

K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. Alternative splicing results in two transcript variants encoding different isoforms. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC12A5