KAT3B/p300 Antibody (1H11G2) - BSA Free

Chromatin Immunoprecipitation: KAT3B/p300 Antibody (1H11G2) [NBP2-59233] - ChIP was performed using HeLa cells, the antibody against p300 and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for two genomic regions near the ANKRD32 and IRS2 genes, used as positive controls, and for the coding region of the inactive MYOD1 gene and an intergeic region on chromosome 11, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

• Reactivity: Hu
• Applications: ChIP
• Clone: 1H11G2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

KAT3B/p300 Antibody (1H11G2) - BSA Free Summary

• Immunogen: The exact immunogen is proprietary information.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: EP300
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 5 ug
  • Chromatin Immunoprecipitation Sequencing

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide and 0.05% ProClin 300
• Purity: Protein A purified

Alternate Names for KAT3B/p300 Antibody (1H11G2) - BSA Free

• E1A binding protein p300
• E1A-associated protein p300
• E1A-binding protein, 300kD
• EC 2.3.1
• EC 2.3.1.48
• EP300
• histone acetyltransferase p300
• KAT3B
• p300 HAT
• p300
• RSTS2

Background

p300 is a relatively stable and ubiquitously expressed nuclear phosphoprotein which is conserved among a variety of mammalian species. It is actively synthesized and phosphorylated in both quiescent and proliferating cells. Cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) and p300 are closely related transcriptional coactivators and have been shown to directly interact with many different DNA-binding transcription factors including nuclear hormone receptors, CREB, c-Fos, C-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD. Both CBP and p300 have been shown to display histone acetyltransferase (HAT) activity, capable of acetylating all four core histone particles in nucleosomes. As a result of HAT activity, it has been suggested CBP and p300 may play a direct role in activating chromatin for transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EP300