Recombinant Human KAP1 His Protein

SDS-Page: KAP1 Protein [NBP2-22889]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human KAP1 His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 366-802 of Human KAP1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSKLIYFQL HRALKMIVDP VEPHGEMKFQ WDLNAWTKSA EAFGKIVAER PGTNSTGPAP MAPPRAPGPL SKQGSGSSQP MEVQEGYGFG SGDDPYSSAE PHVSGVKRSR SGEGEVSGLM RKVPRVSLER LDLDLTADSQ PPVFKVFPGS TTEDYNLIVI ERGAAAAATG QPGTAPAGTP GAPPLAGMAI VKEEETEAAI GAPPTATEGP ETKPVLMALA EGPGAEGPRL ASPSGSTSSG LEVVAPEGTS APGGGPGTLD DSATICRVCQ KPGDLVMCNQ CEFCFHLDCH LPALQDVPGE EWSCSLCHVL PDLKEEDGSL SLDGADSTGV VAKLSPANQR KCERVLLALF CHEPCRPLHQ LATDSTFSLD QPGGTLDLTL IRARLQEKLS PPYSSPQEFA QDVGRMFKQF NKLTEDKADV QSIIGLQRFF ETRMNEAFGD

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TRIM28
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 48.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 0.4M UREA
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human KAP1 His Protein

• E3 SUMO-protein ligase TRIM28
• EC 6.3.2.-
• FLJ29029
• KAP1
• KAP-1
• KAP1KRAB-associated protein 1
• KRIP-1
• Nuclear corepressor KAP-1
• RNF96
• RNF96KRAB-interacting protein 1
• TF1B
• TIF1B
• TIF1-beta
• TIF1BRING finger protein 96
• transcription intermediary factor 1-beta
• transcriptional intermediary factor 1-beta
• TRIM28
• tripartite motif containing 28
• tripartite motif-containing 28
• Tripartite motif-containing protein 28

Background

TRIM28 mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Recombinant human TRIM28 protein, fused to His-tag at N-terminus, was expressed in E.coli .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: TRIM28