KAP1 Antibody

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Immunohistochemistry-Paraffin: KAP1 Antibody [NB100-1161] - 4ug/ml staining Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

KAP1 Antibody Summary

Immunogen
Peptide with sequence C-SSQELSGGPGDGP corresponding to C-Terminus according to NP_005753.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TRIM28
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:4000
Application Notes
IF successful usage on mouse embryo at the late zygote stage, 5ug/ml. ChIP successful usage for K562 cells is reported in scientific literature (PMID: 21170338). WB: Approx. 120 kDa band observed in HepG2 lysates (calculated MW of 88.5 kDa band according to NP_005753.1). The higher observed MW is explained by sumoylated protein. IHC-P: Human breast shows nuclear staining in epithelial duct cells. IHC successful usage on human gastric cancer surgical specimens is reported in scientific literature (PMID: 19898899).
Publications
Read Publications using NB100-1161.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for KAP1 Antibody

  • E3 SUMO-protein ligase TRIM28
  • EC 6.3.2.-
  • FLJ29029
  • KAP1
  • KAP-1
  • KAP1KRAB-associated protein 1
  • KRIP-1
  • Nuclear corepressor KAP-1
  • RNF96
  • RNF96KRAB-interacting protein 1
  • TF1B
  • TIF1B
  • TIF1-beta
  • TIF1BRING finger protein 96
  • transcription intermediary factor 1-beta
  • transcriptional intermediary factor 1-beta
  • TRIM28
  • tripartite motif containing 28
  • tripartite motif-containing 28
  • Tripartite motif-containing protein 28

Background

The KRAB (Kruppel Associated Box) domain is minimally about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors. Over 220 KRAB zinc finger protein (KRAB ZFP) genes have been identified in the human genome. These proteins functionally repress transcription via specific interactions with KAP1 (KRAB Associated Protein 1). KAP1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger. KAP1 has been shown to form complexes with KRAB domain transcription factors and increase the efficiency with which they mediate repression. KAP1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel associated box containing zinc finger protein 1). KAP1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, helping to regulate transcriptional repression. Recent studies have shown that KAP1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for KAP1 Antibody (NB100-1161)(3)

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol TRIM28
Entrez
Uniprot