KAP1 Antibody (821912) [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clone: 821912
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

KAP1 Antibody (821912) [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human KAP1
  Lys266-Ser624
  Accession # Q13263
• Specificity: Detects human KAP1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KAP1 Antibody (821912) [Alexa Fluor® 350]

• E3 SUMO-protein ligase TRIM28
• EC 6.3.2.-
• FLJ29029
• KAP1
• KAP-1
• KAP1KRAB-associated protein 1
• KRIP-1
• Nuclear corepressor KAP-1
• RNF96
• RNF96KRAB-interacting protein 1
• TF1B
• TIF1B
• TIF1-beta
• TIF1BRING finger protein 96
• transcription intermediary factor 1-beta
• transcriptional intermediary factor 1-beta
• TRIM28
• tripartite motif containing 28
• tripartite motif-containing 28
• Tripartite motif-containing protein 28

Background

KAP1 (KRAB-interacting protein 1; gene name TRIM28), also called TIF1b (transcription intermediary factor 1b) is an approximately 100 kDa nuclear corepressor that is a member of the TRIM (tripartite motif-containing) family. It is reported to bind both IRF7 and the SUMO enzyme and cause increased SUMOylation of IRF7. The 834 amino acid (aa) mature protein contains four zinc finger regions and a leucine zipper domain. A 752 aa human splice variant lacking aa 114-195 has been reported. Within the region used as an immunogen, human KAP1 shares 94% aa sequence identity with mouse and rat KAP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q13263(Human)