KAP1 Antibody (821912) [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clone: 821912
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

KAP1 Antibody (821912) [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human KAP1
  Lys266-Ser624
  Accession # Q13263
• Specificity: Detects human KAP1 in direct ELISAs and Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KAP1 Antibody (821912) [Alexa Fluor® 647]

• E3 SUMO-protein ligase TRIM28
• EC 6.3.2.-
• FLJ29029
• KAP1
• KAP-1
• KAP1KRAB-associated protein 1
• KRIP-1
• Nuclear corepressor KAP-1
• RNF96
• RNF96KRAB-interacting protein 1
• TF1B
• TIF1B
• TIF1-beta
• TIF1BRING finger protein 96
• transcription intermediary factor 1-beta
• transcriptional intermediary factor 1-beta
• TRIM28
• tripartite motif containing 28
• tripartite motif-containing 28
• Tripartite motif-containing protein 28

Background

KAP1 (KRAB-interacting protein 1; gene name TRIM28), also called TIF1b (transcription intermediary factor 1b) is an approximately 100 kDa nuclear corepressor that is a member of the TRIM (tripartite motif-containing) family. It is reported to bind both IRF7 and the SUMO enzyme and cause increased SUMOylation of IRF7. The 834 amino acid (aa) mature protein contains four zinc finger regions and a leucine zipper domain. A 752 aa human splice variant lacking aa 114-195 has been reported. Within the region used as an immunogen, human KAP1 shares 94% aa sequence identity with mouse and rat KAP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.