KA2/GRIK5/Glutamate Receptor KA2 Antibody

Immunohistochemistry-Paraffin: KA2/GRIK5/Glutamate Receptor KA2 Antibody [NBP1-36959] - (3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Rt, Bv
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

KA2/GRIK5/Glutamate Receptor KA2 Antibody Summary

• Immunogen: Peptide with sequence C-GPAGPRELAEHE corresponding to C-Terminus according to NP_002079.3.
• Epitope: C-Terminus
• Predicted Species: Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GRIK5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3.75 ug/ml
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 ug/ml
• Application Notes: Western blot: Preliminary experiments gave an approx. 26kDa band in Human Brain (Frontal Cortex and Hippocampus) lysates after 1ug/ml antibody staining. Please note that currently we can not find an explanation in the literature for the band we observe given the calculated size of 109kDa according to NP_002079.3. The 26kDa band was successfully blocked by incubation with the immunizing peptide.
  IHC: Paraffin embedded Human Brain (Cortex).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for KA2/GRIK5/Glutamate Receptor KA2 Antibody

• EAA2
• Excitatory amino acid receptor 2
• GluK5
• glutamate receptor KA2
• Glutamate receptor KA-2
• glutamate receptor, ionotropic kainate 5
• glutamate receptor, ionotropic, kainate 5
• GRIK2
• GRIK5
• KA2/GRIK5
• KA2GRIK2

Background

Glutamate receptor KA2 is a protein that interacts with glutamate, an excitatory neurotransmitter, in the central nervous system and that has two isoforms, measuring 980 and 981 amino acids in length, with weights of approximately 109 and 110 kDa respectively. Current studies are being done on diseases and disorders related to this protein including temporal lobe epilepsy, embryonal carcinoma, Parkinson's disease, schizophrenia, retinoblastoma, neruoblastoma, thyroiditis, and neuronitis. Glutamate receptor KA2 has also been shown to have interactions with GOLM1, GPAA1, LRSAM1, DLG4, and GRID2 in pathways such as the glutamic acid signaling, transmission across chemical synapses, and activation of kainite receptor pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KA2/GRIK5/Glutamate Receptor KA2 Antibody (NBP1-36959)(1)

Publication using NBP1-36959

• Fotheringham J, Williams EL, Akhyani N, Jacobson S. Human herpesvirus 6 (HHV-6) induces dysregulation of glutamate uptake and transporter expression in astrocytes. J Neuroimmune Pharmacol 2008-06-01 [PMID: 18247129]

Bioinformatics

• Gene Symbol: GRIK5
• Entrez:
  • 2901(Human)
  • 24407(Rat)
• Uniprot:
  • NP_002079.3(Human)