Jumonji/JARID2 Antibody

Images

 
Genetic Strategies: Knockout Validated: Jumonji/JARID2 Antibody [NB100-2214] - Jumonji/JARID2 N-terminus mediates direct interaction with H2AK119u1-modified nucleosomes. Immunoblot of nucleosomal pull downs from ...read more
Immunocytochemistry/ Immunofluorescence: Jumonji/JARID2 Antibody [NB100-2214] - JARID2 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Western Blot: Jumonji/JARID2 Antibody [NB100-2214] - Jarid2 expression in naive and activated (overnight) murine B cells. This image was submitted via customer Review.
Immunohistochemistry-Paraffin: Jumonji/JARID2 Antibody [NB100-2214] - Tested in mouse brain using DAB with hematoxylin counterstain.
Western Blot: Jumonji/JARID2 Antibody [NB100-2214] - Detection of JARID2 protein in E16 mouse brain lysate using 2ug/ml of NB100-2214.
Simple Western: Jumonji/JARID2 Antibody [NB100-2214] - Simple Western lane view shows a specific band for JARID2 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity Hu, Mu, RbSpecies Glossary
Applications WB, Simple Western, ChIP, ICC/IF, IHC, IP, ChIP, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Jumonji/JARID2 Antibody Summary

Immunogen
A synthetic peptide made to an N-terminal portion of the human JARID2 protein sequence (between residues 1-100). [UniProt# Q92833]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
JARID2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Chromatin Immunoprecipitation 1:10-1:500. Use reported in scientific literature (PMID 22396653)
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500. Use reported in scientific literature
  • Knockout Validated
  • Simple Western 1:10
  • Western Blot 1:500
Application Notes
By Western blot a band is seen at ~140 kDa. Another band is seen >200 kDa and a faint band may be seen ~80 kDa. In ICC/IF, nuclear staining was observed in HeLa cells. In IHC-P, staining was observed in the nucleus of mouse brain. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control Peptide
Jumonji/JARID2 Peptide (NB100-2214PEP)
Reviewed Applications
Read 1 Review rated 4
using
NB100-2214 in the following applications:

Publications
Read Publications using
NB100-2214 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24074864)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Jumonji/JARID2 Antibody

  • JARID2
  • JMJ
  • JMJjumonji-like protein
  • jumonji (mouse) homolog
  • jumonji homolog
  • Jumonji
  • jumonji, AT rich interactive domain 2
  • Jumonji/ARID domain-containing protein 2
  • protein Jumonji

Background

JARID2 (also known as Jumonji) is a nuclear protein that participates in the negative regulation of cell growth. It is required for neural tube formation and is essential for normal heart development and function. As well, it is a part of a group of proteins characterized by a novel structural motif, the JmjC domain, which is implicated in chromatin regulation through histone demethylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rb
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IP, ChIP, KO

Publications for Jumonji/JARID2 Antibody (NB100-2214)(19)

We have publications tested in 3 confirmed species: Human, Mouse, Rabbit.

We have publications tested in 5 applications: Chemotaxis, ICC/IF, IHC-P, IP, WB.


Filter By Application
Chemotaxis
(6)
ICC/IF
(2)
IHC-P
(1)
IP
(3)
WB
(13)
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Filter By Species
Human
(5)
Mouse
(10)
Rabbit
(1)
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Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NB100-2214 Applications Species
Hickey GJ, Wike CL, Nie X et al. Establishment of developmental gene silencing by ordered polycomb complex recruitment in early zebrafish embryos eLife 2022-01-04 [PMID: 34982026]
Sun Z, Tang Y, Zhang Y Et al. Joint single-cell multiomic analysis in Wnt3a induced asymmetric stem cell division Nature communications 2021-10-12 [PMID: 34642323] (WB, Mouse) WB Mouse
Jain P, Ballare C, Blanco E et Al. PHF19 mediated regulation of proliferation and invasiveness in prostate cancer cells Elife 2020-03-10 [PMID: 32155117] (WB, Human) WB Human
Kumar R, Evans T Activation-Induced Cytidine Deaminase Regulates Fibroblast Growth Factor/Extracellular Signal-Regulated Kinases Signaling To Achieve the Naive Pluripotent State During Reprogramming Stem Cells 2019-04-25 [PMID: 31021461] (WB, Mouse) WB Mouse
Yen YP, Hsieh WF, Tsai YY et al. Dlk1-Dio3 locus-derived lncRNAs perpetuate postmitotic motor neuron cell fate and subtype identity Elife. 2018-10-11 [PMID: 30311912] (WB, IP, Mouse) WB, IP Mouse
Perino M, van Mierlo G, Karemaker ID et al. MTF2 recruits Polycomb Repressive Complex 2 by helical-shape-selective DNA binding Nat. Genet. 2018-05-28 [PMID: 29808031] (Chemotaxis) Chemotaxis
Schneider E, Staffas A, Rohner L et al. MicroRNA-155 is a direct target of Meis1, but not a driver in acute myeloid leukemia. Haematologica 2017-12-07 [PMID: 29217774] (WB) WB
Basu M, Zhu JY, LaHaye S et al. Epigenetic mechanisms underlying maternal diabetes-associated risk of congenital heart disease. JCI Insight. 2017-10-19 [PMID: 29046480] (IHC-P, ICC/IF, WB, Chemotaxis, Mouse) IHC-P, ICC/IF, WB, Chemotaxis Mouse
Qu Y, Yang Q, Liu J et al. c-Myc is Required for BRAF(V600E)-Induced Epigenetic Silencing by H3K27me3 in Tumorigenesis. Theranostics. 2017-06-28 [PMID: 28656062] (WB, Human) WB Human
Jin HY, Oda H, Chen P et al. Differential Sensitivity of Target Genes to Translational Repression by miR-17-92. PLoS Genet. [PMID: 28241004] (WB, Mouse) WB Mouse
Show All 19 Publications.

Review for Jumonji/JARID2 Antibody (NB100-2214) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-2214:
Filter by Applications
WB
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot Jumonji/JARID2 NB100-2214
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4
reviewed by:
Verified Customer
WB Mouse 02/28/2017
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Summary

ApplicationWestern Blot
Sample TestedB cells
SpeciesMouse

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Jumonji/JARID2 Antibody (NB100-2214). (Showing 1 - 1 of 1 FAQs).

  1. What is the WB band size and recommended antibody dilution?
    • We observed a band at approximately 140kDa. The bands ~80 and 200kDa also come up on our blots but we are not sure of their identity. Recommended dilution for WB is 2ug/ml.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Jumonji/JARID2 Antibody (NB100-2214)

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Recent Reviews

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Verified Customer
02/28/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol JARID2
Entrez
Uniprot