Jumonji/JARID2 Antibody

Images

 
Western Blot: Jumonji/JARID2 Antibody [NB100-2214] - Jarid2 expression in naive and activated (overnight) murine B cells. This image was submitted via customer Review.
Immunocytochemistry/ Immunofluorescence: Jumonji/JARID2 Antibody [NB100-2214] - JARID2 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry-Paraffin: Jumonji/JARID2 Antibody [NB100-2214] - Tested in mouse brain using DAB with hematoxylin counterstain.
Western Blot: Jumonji/JARID2 Antibody [NB100-2214] - Detection of JARID2 protein in E16 mouse brain lysate using 2ug/ml of NB100-2214.
Simple Western: Jumonji/JARID2 Antibody [NB100-2214] - Simple Western lane view shows a specific band for JARID2 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity Hu, Mu, RbSpecies Glossary
Applications WB, Simple Western, ChIP, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.03 mg/ml

Jumonji/JARID2 Antibody Summary

Immunogen
A synthetic peptide made to an N-terminal portion of the human JARID2 protein sequence (between residues 1-100). [UniProt# Q92833]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
JARID2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Simple Western 1:10
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
Application Notes
This JARID2 antibody is useful for Immunocytochemistry/Immunofluorescence, Western Blot, and Immunohistochemistry-paraffin embedded sections. Immunoprecipitation was reported in scientific literature. By Western blot a band is seen at ~140 kDa. Another band is seen >200 kDa and a faint band may be seen ~80 kDa. This antibody has also been reported to work in Chromatin Immunoprecipitation (ChIP), see PMID: 22396653. In ICC/IF, nuclear staining was observed in HeLa cells. In IHC-P, staining was observed in the nucleus of mouse brain. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
Jumonji/JARID2 Lysate (NBP2-64847)
Control Peptide
Jumonji/JARID2 Peptide (NB100-2214PEP)
Reviewed Applications
Read 1 Review rated 4
using
NB100-2214 in the following applications:

Publications
Read Publications using
NB100-2214 in the following applications:

  • 6 publications
  • 2 publications
  • 1 publication
  • IP
    2 publications
  • WB
    9 publications

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 24074864)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.03 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Jumonji/JARID2 Antibody

  • JARID2
  • JMJ
  • JMJjumonji-like protein
  • jumonji (mouse) homolog
  • jumonji homolog
  • Jumonji
  • jumonji, AT rich interactive domain 2
  • Jumonji/ARID domain-containing protein 2
  • protein Jumonji

Background

JARID2 (also known as Jumonji) is a nuclear protein that participates in the negative regulation of cell growth. It is required for neural tube formation and is essential for normal heart development and function. As well, it is a part of a group of proteins characterized by a novel structural motif, the JmjC domain, which is implicated in chromatin regulation through histone demethylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-2214
Species: Hu, Mu, Rb
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IHC-P, IP

Publications for Jumonji/JARID2 Antibody (NB100-2214)(14)

We have publications tested in 3 confirmed species: Human, Mouse, Rabbit.

We have publications tested in 5 applications: ChIP, ICC/IF, IHC-P, IP, WB.


Filter By Application
ChIP
(6)
ICC/IF
(2)
IHC-P
(1)
IP
(2)
WB
(9)
All Applications
Filter By Species
Human
(4)
Mouse
(7)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB100-2214 Applications Species
Perino M, van Mierlo G, Karemaker ID et al. MTF2 recruits Polycomb Repressive Complex 2 by helical-shape-selective DNA binding Nat. Genet. May 28 2018 [PMID: 29808031] (ChIP) ChIP
Schneider E, Staffas A, Rohner L et al. MicroRNA-155 is a direct target of Meis1, but not a driver in acute myeloid leukemia. Haematologica 2017 Dec 07 [PMID: 29217774] (WB) WB
Basu M, Zhu JY, LaHaye S et al. Epigenetic mechanisms underlying maternal diabetes-associated risk of congenital heart disease. JCI Insight. 2017 Oct 19 [PMID: 29046480] (ICC/IF, WB, ChIP, IHC-P, Mouse) ICC/IF, WB, ChIP, IHC-P Mouse
Qu Y, Yang Q, Liu J et al. c-Myc is Required for BRAF(V600E)-Induced Epigenetic Silencing by H3K27me3 in Tumorigenesis. Theranostics. 2017 Jun 28 [PMID: 28656062] (WB, Human) WB Human
Jin HY, Oda H, Chen P et al. Differential Sensitivity of Target Genes to Translational Repression by miR-17-92. PLoS Genet. [PMID: 28241004] (WB, Mouse) WB Mouse
Fantini D, Huang S, Asara JM et al. Chromatin Association of XRCC5/6 in the Absence of DNA Damage Depends upon the XPE Gene Product DDB2. Molecular Biology of The Cell. Nov 9 2016 [PMID: 28035050] (WB, Mouse) WB Mouse
Cooper S, Grijzenhout A, Underwood E et al. Jarid2 binds mono-ubiquitylated H2A lysine 119 to mediate crosstalk between Polycomb complexes PRC1 and PRC2. Nat Commun. Nov 28 2016 [PMID: 27892467] (WB, ICC/IF, Human) WB, ICC/IF Human
Yoon SJ, Foley JW, Baker JC. HEB associates with PRC2 and SMAD2/3 to regulate developmental fates. Nat Commun. 2015 Mar 17 [PMID: 25775035] (ChIP, Mouse) ChIP Mouse
Tange Shoichiro, Oktyabri Dulamsuren, Terashima Minoru et al. JARID2 is involved in transforming growth factor-beta-induced epithelial-mesenchymal transition of lung and colon cancer cell lines. PLoS One. 2014 Dec 26 [PMID: 25542019] (WB) WB
Pereira RM, Martinez GJ, Engel I et al. Jarid2 is induced by TCR signalling and controls iNKT cell maturation. Nat Commun. 2014 Aug 09 [PMID: 25105474] (ChIP, Mouse) ChIP Mouse
Show All 14 Publications.

Review for Jumonji/JARID2 Antibody (NB100-2214) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-2214:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Jumonji/JARID2 NB100-2214
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reviewed by:
Hyun Yong Jin
WB Mouse 02/28/2017
View

Summary

ApplicationWestern Blot
Sample TestedB cells
SpeciesMouse

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Jumonji/JARID2 Antibody (NB100-2214). (Showing 1 - 1 of 1 FAQs).

  1. What is the WB band size and recommended antibody dilution?
    • We observed a band at approximately 140kDa. The bands ~80 and 200kDa also come up on our blots but we are not sure of their identity. Recommended dilution for WB is 2ug/ml.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-2214AF405
Alexa Fluor 488 Labeled NB100-2214AF488
Alexa Fluor 647 Labeled NB100-2214AF647
Alexa Fluor 700 Labeled NB100-2214AF700
Biotin Labeled NB100-2214B
DyLight 405 Labeled NB100-2214V
DyLight 488 Labeled NB100-2214G
DyLight 550 Labeled NB100-2214R
DyLight 650 Labeled NB100-2214C
FITC Labeled NB100-2214F
HRP Labeled NB100-2214H

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Recent Reviews

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Hyun Yong Jin
02/28/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol JARID2
Entrez
Uniprot