Jumonji/JARID2 Antibody


Western Blot: Jumonji/JARID2 Antibody [NB100-2214] - Detection of JARID2 protein in E16 mouse brain lysate using 2ug/ml of NB100-2214.
Immunocytochemistry/ Immunofluorescence: Jumonji/JARID2 Antibody [NB100-2214] - JARID2 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry-Paraffin: Jumonji/JARID2 Antibody [NB100-2214] - Tested in mouse brain using DAB with hematoxylin counterstain.
Simple Western: Jumonji/JARID2 Antibody [NB100-2214] - Simple Western lane view shows a specific band for JARID2 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Reactivity Hu, Mu, RbSpecies Glossary
Applications WB, Simple Western, ChIP, ICC/IF, IHC, IHC-P, IP
1.03 mg/ml

Jumonji/JARID2 Antibody Summary

A synthetic peptide made to an N-terminal portion of the human JARID2 protein sequence (between residues 1-100). [UniProt# Q92833]
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
1.03 mg/ml
Immunogen affinity purified


  • Western Blot 2 ug/ml
  • Simple Western 1:10
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
Application Notes
This JARID2 antibody is useful for Immunocytochemistry/Immunofluorescence, Western Blot, and Immunohistochemistry-paraffin embedded sections. Immunoprecipitation was reported in scientific literature. By Western blot a band is seen at ~140 kDa. Another band is seen >200 kDa and a faint band may be seen ~80 kDa. This antibody has also been reported to work in Chromatin Immunoprecipitation (ChIP), see PMID: 22396653. In ICC/IF, nuclear staining was observed in HeLa cells. In IHC-P, staining was observed in the nucleus of mouse brain. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Control Peptide
Jumonji/JARID2 Peptide (NB100-2214PEP)
Read Publications using
NB100-2214 in the following applications:

  • 4 publications
  • IP
    2 publications
  • WB
    3 publications

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 24074864)

Alternate Names for Jumonji/JARID2 Antibody

  • JARID2
  • JMJ
  • JMJjumonji-like protein
  • jumonji (mouse) homolog
  • jumonji homolog
  • jumonji, AT rich interactive domain 2
  • Jumonji/ARID domain-containing protein 2
  • protein Jumonji


JARID2 (also known as Jumonji) is a nuclear protein that participates in the negative regulation of cell growth. It is required for neural tube formation and is essential for normal heart development and function. As well, it is a part of a group of proteins characterized by a novel structural motif, the JmjC domain, which is implicated in chromatin regulation through histone demethylation.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Jumonji/JARID2 Antibody (NB100-2214)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Rabbit.

We have publications tested in 3 applications: ChIP, IP, WB.

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Showing Publications 1 - 7 of 7.
Publications using NB100-2214 Applications Species
Yoon SJ, Foley JW, Baker JC. HEB associates with PRC2 and SMAD2/3 to regulate developmental fates. Nat Commun. 2015 Mar 17 [PMID:25775035] (ChIP, Mouse) ChIP Mouse
Tange Shoichiro, Oktyabri Dulamsuren, Terashima Minoru et al. JARID2 is involved in transforming growth factor-beta-induced epithelial-mesenchymal transition of lung and colon cancer cell lines. PLoS One. 2014 Dec 26 [PMID:25542019] (WB) WB
Pereira RM, Martinez GJ, Engel I et al. Jarid2 is induced by TCR signalling and controls iNKT cell maturation. Nat Commun. 2014 Aug 09 [PMID:25105474] (ChIP, Mouse) ChIP Mouse
Banaszynski LA, Wen D, Dewell S et al. Hira-Dependent Histone H3.3 Deposition Facilitates PRC2 Recruitment at Developmental Loci in ES Cells. Cell. 2013 Sep 26 [PMID:24074864] (ChIP, WB, Mouse, Rabbit) ChIP, WB Mouse, Rabbit
Holdt LM, Hoffmann S, Sass K et al. Alu Elements in ANRIL Non-Coding RNA at Chromosome 9p21 Modulate Atherogenic Cell Functions through Trans-Regulation of Gene Networks. PLoS Genet 2013 Jul [PMID:23861667] (IP, Human) IP Human
Dietrich N, Lerdrup M, Landt E et al. REST-Mediated Recruitment of Polycomb Repressor Complexes in Mammalian Cells PLoS Genet 2012 [PMID:22396653] (ChIP, Mouse) ChIP Mouse
Shirato H, Ogawa S, Nakajima K et al. A Jumonji (Jarid2) Protein Complex Represses cyclin D1 Expression by Methylation of Histone H3-K9. J Biol Chem 2009;284(2):733-739. [PMID:19010785] (WB, IP, Human) WB, IP Human

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Jumonji/JARID2 Antibody (NB100-2214) (0)

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB100-2214AF405
Alexa Fluor (R) 488 Labeled NB100-2214AF488
Alexa Fluor (R) 647 Labeled NB100-2214AF647
Alexa Fluor (R) 700 Labeled NB100-2214AF700
Biotin Labeled NB100-2214B
DyLight 405 Labeled NB100-2214V
DyLight 488 Labeled NB100-2214G
DyLight 550 Labeled NB100-2214R
DyLight 650 Labeled NB100-2214C
FITC Labeled NB100-2214F
HRP Labeled NB100-2214H

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Contact Information

Product PDFs


Gene Symbol JARID2

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