Jumonji/JARID2 Antibody - BSA Free

Western Blot: Jumonji/JARID2 Antibody [NB100-2214] - Jumonji/JARID2 N-terminus mediates direct interaction with H2AK119u1-modified nucleosomes. Immunoblot of nucleosomal pull downs from nuclear extract. Total nuclear extract (input) and pull down using streptavidin beads, unmodified nucleosomes or nucleosomes modified by H2AK119u1/H2AK119u1(I44A) were probed with the indicated antibodies using extracts from WT cells (left) or Jumonji/JARID2 KO mESCs (right). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms13661) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Jumonji/JARID2 Antibody [NB100-2214] - JARID2 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Western Blot: Jumonji/JARID2 Antibody [NB100-2214] - Jarid2 expression in naive and activated (overnight) murine B cells. This image was submitted via customer Review.

Immunohistochemistry-Paraffin: Jumonji/JARID2 Antibody [NB100-2214] - Tested in mouse brain using DAB with hematoxylin counterstain.

Western Blot: Jumonji/JARID2 Antibody [NB100-2214] - Detection of JARID2 protein in E16 mouse brain lysate using 2ug/ml of NB100-2214.

Simple Western: Jumonji/JARID2 Antibody [NB100-2214] - Simple Western lane view shows a specific band for JARID2 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Western Blot: Jumonji/JARID2 Antibody [NB100-2214] - JARID2 N-terminus mediates direct interaction with H2AK119u1-modified nucleosomes(a) Immunoblot of nucleosomal pull downs from nuclear extract. Total nuclear extract (input) & pull down using streptavidin beads, unmodified nucleosomes or nucleosomes modified by H2AK119u1/H2AK119u1(I44A) were probed with the indicated antibodies using extracts from WT cells (left) or JARID2 KO mESCs (right). Full blots are shown in Supplementary Fig. 9. (b) JARID2 1–530 binds to H2AK119u1-modified mononucleosomes (yellow) more tightly than unmodified (blue) or H2AK199u1(I44A) (red) as monitored by biolayer interferometry (n=3). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27892467), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rb
• Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IP, ChIP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Jumonji/JARID2 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to an N-terminal portion of the human JARID2 protein sequence (between residues 1-100). [UniProt# Q92833]
• Localization: Nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: JARID2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Chromatin Immunoprecipitation 1:10-1:500. Use reported in scientific literature (PMID 22396653)
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500. Use reported in scientific literature
  • Knockout Validated
  • Simple Western 1:10
  • Western Blot 1:500
• Application Notes: By Western blot a band is seen at ~140 kDa. Another band is seen >200 kDa and a faint band may be seen ~80 kDa. In ICC/IF, nuclear staining was observed in HeLa cells. In IHC-P, staining was observed in the nucleus of mouse brain. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in Human Brain lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:10, apparent MW was 128 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Control Peptide:

  Jumonji/JARID2 Peptide (NB100-2214PEP)

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24074864)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Jumonji/JARID2 Antibody - BSA Free

• JARID2
• JMJ
• JMJjumonji-like protein
• jumonji (mouse) homolog
• jumonji homolog
• Jumonji
• jumonji, AT rich interactive domain 2
• Jumonji/ARID domain-containing protein 2
• protein Jumonji

Background

JARID2 (also known as Jumonji) is a nuclear protein that participates in the negative regulation of cell growth. It is required for neural tube formation and is essential for normal heart development and function. As well, it is a part of a group of proteins characterized by a novel structural motif, the JmjC domain, which is implicated in chromatin regulation through histone demethylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Jumonji/JARID2 Antibody (NB100-2214)(26)

Publications using NB100-2214

• Arecco N, Mocavini I, Blanco E, Ballaré C et Al. Alternative splicing decouples local from global PRC2 activity Mol Cell 2024-03-07 [PMID: 38452766]
• Liu W, Zeng Y, Hao X et al. JARID2 coordinates with the NuRD complex to facilitate breast tumorigenesis through response to adipocyte-derived leptin Cancer communications (London, England) 2023-09-01 [PMID: 37658635] (ICC/IF, IHC-P, WB, ChIP, Human)
• Kate Skehan, Matthew Richardson, Laura M O'Connor, Samuel Dickson, Kate Martin, Geetha Govindarajulu, Swetha Sridharan Viscous Aqueous Gel Illustrating Natural Anatomy: The VAGINA method in gynaecological MRI simulation. Journal of medical radiation sciences 2023-08-24 [PMID: 37621131]
• Kadomatsu T, Hara C, Kurahashi R et al. ANGPTL2-mediated epigenetic repression of MHC-I in tumor cells accelerates tumor immune evasion Molecular oncology 2023-07-15 [PMID: 37452654] (IHC-Fr, Mouse)
• Hickey GJ, Wike CL, Nie X et al. Establishment of developmental gene silencing by ordered polycomb complex recruitment in early zebrafish embryos eLife 2022-01-04 [PMID: 34982026]
• Sun Z, Tang Y, Zhang Y Et al. Joint single-cell multiomic analysis in Wnt3a induced asymmetric stem cell division Nature communications 2021-10-12 [PMID: 34642323] (WB, Mouse)
• Matteo Perino, Guido van Mierlo, Chet Loh, Sandra M.T. Wardle, Dick W. Zijlmans, Hendrik Marks, Gert Jan C. Veenstra Two Functional Axes of Feedback-Enforced PRC2 Recruitment in Mouse Embryonic Stem Cells Stem Cell Reports 2020-08-06 [PMID: 32763159]
• Jain P, Ballare C, Blanco E et Al. PHF19 mediated regulation of proliferation and invasiveness in prostate cancer cells Elife 2020-03-10 [PMID: 32155117] (WB, Human)
• Kumar R, Evans T Activation-Induced Cytidine Deaminase Regulates Fibroblast Growth Factor/Extracellular Signal-Regulated Kinases Signaling To Achieve the Naive Pluripotent State During Reprogramming Stem Cells 2019-04-25 [PMID: 31021461] (WB, Mouse)
• Yen YP, Hsieh WF, Tsai YY et al. Dlk1-Dio3 locus-derived lncRNAs perpetuate postmitotic motor neuron cell fate and subtype identity Elife. 2018-10-11 [PMID: 30311912] (WB, IP, Mouse)
• Perino M, van Mierlo G, Karemaker ID et al. MTF2 recruits Polycomb Repressive Complex 2 by helical-shape-selective DNA binding Nat. Genet. 2018-05-28 [PMID: 29808031] (Chemotaxis)
• Schneider E, Staffas A, Rohner L et al. MicroRNA-155 is a direct target of Meis1, but not a driver in acute myeloid leukemia. Haematologica 2017-12-07 [PMID: 29217774] (WB)
• Basu M, Zhu JY, LaHaye S et al. Epigenetic mechanisms underlying maternal diabetes-associated risk of congenital heart disease. JCI Insight. 2017-10-19 [PMID: 29046480] (IHC-P, ICC/IF, WB, Chemotaxis, Mouse)
• Qu Y, Yang Q, Liu J et al. c-Myc is Required for BRAF(V600E)-Induced Epigenetic Silencing by H3K27me3 in Tumorigenesis. Theranostics. 2017-06-28 [PMID: 28656062] (WB, Human)
• Jin HY, Oda H, Chen P et al. Differential Sensitivity of Target Genes to Translational Repression by miR-17-92. PLoS Genet. [PMID: 28241004] (WB, Mouse)
• Fantini D , Huang S, Asara JM et al. Chromatin Association of XRCC5/6 in the Absence of DNA Damage Depends upon the XPE Gene Product DDB2. Molecular Biology of The Cell. 2016-11-09 [PMID: 28035050] (WB, Mouse)
• Cooper S, Grijzenhout A, Underwood E et al. Jarid2 binds mono-ubiquitylated H2A lysine 119 to mediate crosstalk between Polycomb complexes PRC1 and PRC2. Nat Commun. 2016-11-28 [PMID: 27892467] (ICC/IF, WB, Human)
• Takeshi Suzuki MEG3 long noncoding RNA contributes to the epigenetic regulation of epithelial-mesenchymal transition in lung cancer cell lines J. Biol. Chem., 2016-11-16;0(0):. 2016-11-16 [PMID: 27852821]
• Yoon SJ, Foley JW, Baker JC. HEB associates with PRC2 and SMAD2/3 to regulate developmental fates. Nat Commun. 2015-03-17 [PMID: 25775035] (Chemotaxis, Mouse)
• Tange Shoichiro, Oktyabri Dulamsuren, Terashima Minoru et al. JARID2 is involved in transforming growth factor-beta-induced epithelial-mesenchymal transition of lung and colon cancer cell lines. PLoS One. 2014-12-26 [PMID: 25542019] (WB)
• Pereira RM, Martinez GJ, Engel I et al. Jarid2 is induced by TCR signalling and controls iNKT cell maturation. Nat Commun. 2014-08-09 [PMID: 25105474] (Chemotaxis, Mouse)
• Banaszynski LA, Wen D, Dewell S et al. Hira-Dependent Histone H3.3 Deposition Facilitates PRC2 Recruitment at Developmental Loci in ES Cells. Cell. 2013-09-26 [PMID: 24074864] (WB, Chemotaxis, Rabbit, Mouse)
• Holdt LM, Hoffmann S, Sass K et al. Alu Elements in ANRIL Non-Coding RNA at Chromosome 9p21 Modulate Atherogenic Cell Functions through Trans-Regulation of Gene Networks. PLoS Genet 2013-07-01 [PMID: 23861667] (IP, Human)
• Dietrich N, Lerdrup M, Landt E et al. REST-mediated recruitment of polycomb repressor complexes in mammalian cells. PLoS Genet 2012-01-01 [PMID: 22396653] (Chemotaxis, Mouse)
• Nicola Reynolds, Mali Salmon-Divon, Heidi Dvinge, Antony Hynes-Allen, Gayan Balasooriya, Donna Leaford, Axel Behrens, Paul Bertone, Brian Hendrich NuRD-mediated deacetylation of H3K27 facilitates recruitment of Polycomb Repressive Complex 2 to direct gene repression The EMBO Journal 2012-02-01 [PMID: 22139358]
• Shirato H, Ogawa S, Nakajima K et al. A Jumonji (Jarid2) Protein Complex Represses cyclin D1 Expression by Methylation of Histone H3-K9. J Biol Chem 2009;284(2):733-739. [PMID: 19010785] (IP, WB, Human)

Review for Jumonji/JARID2 Antibody (NB100-2214) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Hyun Yong Jin WB Mouse 02/28/2017

Summary

• Application: Western Blot
• Sample Tested: B cells
• Species: Mouse

FAQs for Jumonji/JARID2 Antibody (NB100-2214). (Showing 1 - 1 of 1 FAQs).

1. What is the WB band size and recommended antibody dilution?
   • We observed a band at approximately 140kDa. The bands ~80 and 200kDa also come up on our blots but we are not sure of their identity. Recommended dilution for WB is 2ug/ml.

Bioinformatics

• Gene Symbol: JARID2
• Entrez:
  • 3720(Human)
  • 16468(Mouse)
• Uniprot:
  • Q92833(Human)