JNK1/JNK2 Antibody

Western Blot: JNK1/JNK2 Antibody [NBP3-13296] - Various whole cell and tissue extracts were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1/JNK2 antibody (NBP3-13296) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: JNK1/JNK2 Antibody [NBP3-13296] - JNK1/JNK2 antibody detects JNK1/JNK2 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. JNK1/JNK2 stained by JNK1/JNK2 antibody (NBP3-13296) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 + JNK2 antibody (NBP3-13296) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Rt, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

JNK1/JNK2 Antibody Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human JNK1/JNK21 + JNK1/JNK22. The exact sequence is proprietary.
• Predicted Species: Mouse (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAPK8
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Reactivity Notes

Xenopus laevis (85%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for JNK1/JNK2 Antibody

• c-Jun N-terminal kinase 1
• JNK
• JNK1
• JNK1/JNK2
• JNK1A2
• JNK21B1/2
• JNK-46
• JUN N-terminal kinase
• MAP kinase 8
• mitogen-activated protein kinase 8
• PRKM8
• SAPK1
• SAPK1C
• Stress-activated protein kinase 1
• stress-activated protein kinase 1c

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPK8