JNK1/JNK2 Antibody (252323) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat JNK1/JNK2 Monoclonal Antibody (Catalog # MAB2076) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human JNK1, JNK2, and JNK3 (1 ng/lane) were included. Specific bands for JNK1 and JNK2 were detected at approximately 46 kDa and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

JNK1/JNK2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat JNK1/JNK2 Monoclonal Antibody (Catalog # MAB2076) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: 252323
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

JNK1/JNK2 Antibody (252323) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human JNK1 isoform 2
• Specificity: Detects human, mouse, and rat p46 and p54 JNK1/JNK2. Does not detect recombinant JNK3.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.2 ug/mL
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for JNK1/JNK2 Antibody (252323) [Unconjugated]

• JNK1/JNK2

Background

The c-Jun N-terminal kinases (JNKs) are encoded by three genes: JNK1, JNK2, and JNK3. Members of the MAPK superfamily, JNKs are activated by environmental stresses and inflammatory cytokines. JNK1, also known as SAPK1 gamma and MAPK8, is expressed as four isoforms generated by alternative splicing. JNK1 is activated by dual phosphorylation at T183 and Y185 by the MAPK kinases MKK4 and/or MKK7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for JNK1/JNK2 Antibody (MAB2076)(4)

Publications using MAB2076

• YY Liu, SY Wang, YN Li, WJ Bian, LQ Zhang, YH Li, L Long, X Liu, XW Zhang, ZG Li Activity of fibroblast-like synoviocytes in rheumatoid arthritis was impaired by dickkopf-1 targeting siRNA Chin. Med. J., 2020;0(6):679-686. 2020 [PMID: 32068606] (Western Blot, Human)
• M Fang, WH Zhong, WL Song, YY Deng, DM Yang, B Xiong, HK Zeng, HD Wang Ulinastatin Ameliorates Pulmonary Capillary Endothelial Permeability Induced by Sepsis Through Protection of Tight Junctions via Inhibition of TNF-? and Related Pathways Front Pharmacol, 2018;9(0):823. 2018 [PMID: 30150933] (Western Blot, Rat)
• S Janczar, J Nautiyal, Y Xiao, E Curry, M Sun, E Zanini, AJ Paige, H Gabra WWOX sensitises ovarian cancer cells to paclitaxel via modulation of the ER stress response Cell Death Dis, 2017;8(7):e2955. 2017 [PMID: 28749468] (Western Blot, Human)
• Chavez-Galan L, Ramon-Luing L, Torre-Bouscoulet L, Perez-Padilla R, Sada-Ovalle I Pre-exposure of Mycobacterium tuberculosis-infected macrophages to crystalline silica impairs control of bacterial growth by deregulating the balance between apoptosis and necrosis. PLoS ONE, 2013;8(11):e80971. 2013 [PMID: 24278357] (Western Blot, Human)