JNK1 Antibody (228611)

Images

 
Western blot shows lysates of CHP-100 human neuroblastoma cell line, C6 rat glioma cell line, TS1 mouse helper T cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL Mouse ...read more
Western blot shows recombinant human JNK1, JNK2, and JNK3 (2 ng/lane). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB1776) followed by HRP-conjugated Anti-Mouse ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
228611
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

JNK1 Antibody (228611) Summary

Immunogen
E. coli-derived recombinant human JNK1 isoform 2
Met1-Gln384
Accession # NP_002741
Specificity
Detects human, mouse, and rat JNK1, expressed predominantly as p46 JNK (isoforms 2 and/or 3, both 384 aa), with lesser amounts of p54 JNK (isoforms 1 and/or 4, both 427 aa), in Western blots. Does not detect recombinant JNK2 or JNK3.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MAPK8
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Reviewed Applications
Read 2 Reviews rated 5
using
MAB1776 in the following application:

Publications
Read Publication using
MAB1776 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for JNK1 Antibody (228611)

  • c-Jun N-terminal kinase 1
  • EC 2.7.11
  • EC 2.7.11.24
  • JNK1 alpha protein kinase
  • JNK1 beta protein kinase
  • JNK1
  • JNK1JNK1A2
  • JNK-46
  • JUN N-terminal kinase
  • MAP kinase 8
  • MAPK 8
  • MAPK8
  • mitogen-activated protein kinase 8 isoform JNK1 alpha1
  • mitogen-activated protein kinase 8 isoform JNK1 beta2
  • mitogen-activated protein kinase 8
  • PRKM8
  • PRKM8JNK
  • protein kinase JNK1
  • SAPK1
  • SAPK1JNK21B1/2
  • Stress-activated protein kinase 1
  • Stress-activated protein kinase JNK1

Background

The c-Jun N-terminal kinases (JNKs) are encoded by three genes: JNK1, JNK2, and JNK3. Members of the MAPK superfamily, JNKs are activated by environmental stresses and inflammatory cytokines. JNK1, also known as SAPK1 gamma and MAPK8, is expressed as four isoforms generated by alternative splicing. JNK1 is activated by dual phosphorylation at T183 and Y185 by the MAPK kinases MKK4 and/or MKK7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for JNK1 Antibody (MAB1776)(1)

We have publications tested in 1 confirmed species: Hamster.

We have publications tested in 1 application: Western Blot.


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Western Blot
(1)
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Reviews for JNK1 Antibody (MAB1776) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Human, Rat, Mouse.

Reviews using MAB1776:
Filter by Applications
WB
(2)
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Filter by Species
Human
(1)
Human, Rat, Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot JNK1 MAB1776
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reviewed by:
Anonymous
WB Human, Rat, Mouse 12/29/2016
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Summary

ApplicationWestern Blot
Sample Testedwhole eye lysate,Retina
SpeciesHuman, Rat, Mouse

Comments

CommentsJNK1 antibody was used overnight at 1:2500, followed by peroxidase conjugated 2nd antibody and chemiluminescence detection
Western Blot JNK1 MAB1776
Enlarge
reviewed by:
Anonymous
WB Human 10/04/2015
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Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell whole cell lysate
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:2000, overnight

Secondary Antibody

Secondary DescriptionSheep anti-mouse IgG, HRP
Secondary Manufacturer Cat#GE Healthcare, NA931V
Secondary Concentration1:2000

Details

Detection NotesRegular Western blot Protocol

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for JNK1 Antibody (MAB1776) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for JNK1 Antibody (MAB1776)

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Blogs on JNK1.

MAPK8/JNK1 - A multifunctional kinase and drug target for cancer therapeutics
The c-Jun N-terminal kinase (JNK) family is a group of regulatory kinases with important functions in cell morphogenesis, inflammation, differentiation, and cell death (1). Aberrant activation of JNK family proteins in cancers has led to interest i...  Read full blog post.

Use Of c-Myc Antibodies In Non-Invasive Cancer Studies
Novus Biologicals specializes in offering top quality antibody reagents to many of the transcription factor proteins that are widely expressed in cancer cells.C-Myc is one of several transcription factor proteins covered by our antibody catalog. Enc...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
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Anonymous
12/29/2016
Application: WB
Species: Human, Rat, Mouse

Anonymous
10/04/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol MAPK8
Uniprot