JMJD1B Antibody

Images

 
Western Blot: JMJD1B Antibody [NB110-38879] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB110-38879 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) ...read more
Immunohistochemistry: JMJD1B Antibody [NB110-38879] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- JMJD1B used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: JMJD1B Antibody [NB110-38879] - FFPE section of human prostate adenocarcinoma. Affinity purified rabbit anti-JMJD1B used at a dilution of 1:250.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

JMJD1B Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1710 and the C-terminus (residue1761) of human Jumonji Domain Containing 1B using the numbering given in entry NP_057688.2 (GeneID 51780).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDM3B
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Positive Control
HeLa Lysate (NB800-PC1)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for JMJD1B Antibody

  • C5orf7
  • EC 1.14.11
  • EC 1.14.11.-
  • JHDM2B
  • JmjC domain-containing histone demethylation protein 2B
  • JMJD1B
  • jumonji domain containing 1B
  • Jumonji domain-containing protein 1B
  • KIAA1082chromosome 5 open reading frame 7
  • lysine (K)-specific demethylase 3B
  • lysine-specific demethylase 3B
  • NET22,5qNCA
  • Nuclear protein 5qNCA

Background

JMJD1B is a member of the jumonji (jmj) domain containing gene family of histone demethylases that plays a role in chromatin regulation and influences transcriptional activation and suppression. Some recently characterized members of the jmj family include JARID1A/RBP2, JARID1C, JMJD1A, JMJD1B, JMJD1C, JMJD2A, JMJD2C and JMJD2D. JMJD1B was identified as the 5qNCA gene that lies within a locus on chromosome 5 that is frequently deleted in myeloid leukemias and myelodysplasias. JMJD1B has been demonstrated to have growth suppressive activity and is implicated as a tumor suppressor gene. Alternate names for JMJD1B include JmjC domain-containing histone demethylation protein 2B, jumonji domain-containing protein 1B, nuclear protein 5qNCA, c5orf7, JHDM2B, and KIAA1082.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol KDM3B
Entrez
Uniprot